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pAAV-CAG-mNeonGreen

Plasmid

#99134

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mNeonGreen

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-tdTomato

Plasmid

#99116

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein tdTomato

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

DIO-tdTomato

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pYFJ16-LplA(W37V) (for E. Coli expression)

Plasmid

#34838

Depositor

Alice Ting

Article

Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

Insert

LplA W37V (lplA E. Coli)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Mutated Trp37 to Val

Promoter

T5 promoter

Available since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-hRIP3 dRHIM

Plasmid

#208788

Purpose

Inducibly expresses human RIP3, lacking RHIM domain, fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

RIP3 (RIPK3 Human)

Use

Lentiviral

Tags

Cry2 mCherry

Expression

Mammalian

Mutation

RHIM domain is removed

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(3,5)P2 Probe

Plasmid

#211512

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3,5-Bisphosphate (PI(3,5)P2) from bacteria

Depositor

David Murray, Hannes Maib

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

SnxA-2xPX

Use

Tags

6xhis-SNAP

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2 HRS-RFP

Plasmid

#29685

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

hepatocyte growth factor-regulated tyrosine kinase substrate (HGS Human)

Use

Tags

RFP

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pGCP123-EbpA_g1

Plasmid

#153517

Purpose

sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

Depositor

Kimberly Kline

Article

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

pnisA-sgRNA(EbpA_g1)-dCas9 scaffold

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLX304 CMV FKBP-V5-sTurboID-high affinity (N)

Plasmid

#153004

Purpose

Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID-high affinity (N) (G78)

Use

Lentiviral

Tags

FKBP and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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mCer-TG2-YFP

Plasmid

#85471

Purpose

TG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRET

Depositor

Ray Truant, Jeffrey Keillor

Article

Caron et al PLoS One. 2012;7(8):e44159. doi: 10.1371

Insert

hTG2 (TGM2 Human)

Use

Tags

EYFP and mCerulean

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pAF0689 Ef1a-Pa01_attB-BFP acceptor

Plasmid

#193472

Purpose

Acceptor plasmid for 3 plasmid recombination assay

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01_attB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LentiGuide_DKO

Plasmid

#183193

Purpose

Plasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

Depositor

Lang Li

Article

Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6, mU6

Available since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pPET-PKR/PPase

Plasmid

#42934

Depositor

James Cole

Article

Lemaire et al J Mol Biol. 2005 Jan 7;345(1):81-90.

Inserts

protein kinase R (EIF2AK2 Human)
phage lambda phosphatase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 12, 2013

Availability

Academic Institutions and Nonprofits only

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FLHKI-pGFPN3

Plasmid

#21917

Depositor

Hossein Ardehali

Article

Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

Insert

Full length human HKI in pGFP-N3 (HK1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

This is full length human HKI in pGFP-N3

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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CAG-NLS-GFP

Plasmid

#104061

Purpose

AAV expression of nuclear localized GFP with SV40 NLS

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has Service

AAV PHP.eB

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-NFE2L2-Puro

Plasmid

#181919

Purpose

Lentiviral plasmid that directs the expression of NFE2L2/Nrf2 in mammalian cells.

Depositor

Scott Dixon

Article

Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

NFE2L2 (NFE2L2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGWB502

Plasmid

#74844

Purpose

Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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Cdk2-HA

Plasmid

#1884

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk2 (CDK2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mTurquoise2

Plasmid

#99122

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mTurquoise2 from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mTurquoise2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pAF0036 Ef1a-Si74-2a-EGFP

Plasmid

#193463

Purpose

Expression of Recombinase

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Si74

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2 8xSTAR-mNeonGreen-NLS. PGK-puro

Plasmid

#136262

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 8 repeats

Use

Tol2 transposase

Tags

mNeonGreen-NLS

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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