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Plasmid#96963PurposeMammalian expressiom of Clontech rtTA driven by human beta-actine promoterDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2017AvailabilityAcademic Institutions and Nonprofits only
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pBad-sfGFP 150TAG
Plasmid#85483PurposeArabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150DepositorInsertsuperfolder GFP 150TAG
UseTagsExpressionBacterialMutation150TAG (amino acid 150 mutated to stop codon)PromoterArabinoseAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFD4-U6:1_U6:3tandemgRNAs
Plasmid#49411Purposeexpression of two gRNAs from Drosophila U6:1 and U6:3DepositorInsertsdU6-1:gRNA
dU6-3:gRNA
UseCRISPRTagsExpressionInsectMutationPromoterdU6-1 and dU6-3Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-GCaMP6f (AAV1)
Viral Prep#135632-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-S5E2-GCaMP6f (#135632). In addition to the viral particles, you will also receive purified pAAV-S5E2-GCaMP6f plasmid DNA. Expression of GCaMP6f under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsNoneAvailable sinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5FRT/TO_HA-mAID-nanobody
Plasmid#117713PurposeExpression of tetracycline-inducible HA-tagged lysine-less mAID fused to lysine-less vhhGFP4 (mAID-nanobody)DepositorInsertmAID-nanobody
UseTagstriple HAExpressionMammalianMutationcodon optimized mAID for murine expressionPromoterCMVAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCCM’
Plasmid#162709PurposepCCM reconstructed after selection for CCMB1 growth on glycerol under ambient airDepositorInsertSecond CCM operon cloned from H. neapolitanus
UseTagsExpressionBacterialMutationp15A origin replaced with high-copy colE1 originPromoterPLtet0-1 promoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330-EN1201
Plasmid#92144PurposeFor transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locusDepositorInsertspCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDAC439
Plasmid#195237PurposeMammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)DepositorInsertFLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pATetO 6XHis-Taq DNA Ligase
Plasmid#178798PurposeInducible expression of Taq DNA Ligase in E. coliDepositorInsertTaq DNA Ligase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_His-CBD-SpEndoH
Plasmid#218196PurposeBacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoHDepositorInsertEndoH
UseTagstufA-His-CBDExpressionBacterialMutationcodon optimized Ecoli, residues 45-313PromoterT7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-HPV66 L1L2
Plasmid#52602PurposeExpresses HPV66 L1 and L2 in mammalian cellsDepositorInsertsHPV66 L1
HPV66 L2
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-Lysosomes-20
Plasmid#55308PurposeLocalization: Lysosome Membrane, Excitation: 399, Emission: 456DepositorInsertLAMP1
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti PGK GFP Blast (w510-5)
Plasmid#19069Purpose3rd gen lentiviral eGFP expression vector, PGK promoter, BlastDepositorInsertenhanced green fluorescent protein
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.eGFP.2A.wtTau
Plasmid#140424PurposeAAV.CBA.eGFP.2A.wtTau propagation reporter WT-2N4R-hTauDepositorInserteGFP-2A-Tau (MAPT Synthetic, Human)
UseAAVTagseGFPExpressionMutationPromoterCBAAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A
Plasmid#86674PurposeExpresses catalytically-inactive GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.DepositorInsertGFP-FLAG-cGAS E225A/D227A
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVoPo-03
Plasmid#187838PurposeBackbone for inducible expression system in Fusobacterium nucleatumDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFPV25.1
Plasmid#20668PurposeS. typhimurium rpsM promoter driving gfpmut3 expression in bacteria. Promoter is not regulated by acidic conditions.DepositorTypeEmpty backboneUseTagsGFP mut3ExpressionBacterialMutationPromoterAvailable sinceMarch 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
PB_iMYOD1_P2A_GFP_Puro
Plasmid#168804PurposePiggyBac transposon construct with doxycycline inducible MYOD1 + GFP expressionDepositorInsertMYOD1 (MYOD1 Synthetic, Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Cre-P2A-dTomato (AAV5)
Viral Prep#107738-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsdTomato (physically separate, not a fusion protein)Available sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-LUC2CP/intron/ARE
Plasmid#62858Purposesplicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.DepositorInsertLuciferase
UseLuciferaseTagsExpressionMammalianMutationinsertion of chimeric intron at nueotide poclsiti…PromoterCMVAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only