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Plasmid#22909DepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2010AvailabilityAcademic Institutions and Nonprofits only
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Orange Nano-lantern(Ca2+)/pcDNA3
Plasmid#71346PurposeExpress Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cellDepositorInsertOrange Nano-lantern(Ca2+)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMBP-LbCas12a
Plasmid#113431PurposeExpresses 10xHis-MBP fused to LbCas12aDepositorHas ServiceCloning Grade DNAInsertLbCas12a
UseTagsMBPExpressionBacterialMutationPromoterT7Available sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
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qTAG-C-mNeon-Blast
Plasmid#207709PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Blast cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG/ATP10A-HA
Plasmid#209215PurposeMammalian expression of ATP10ADepositorInsertATP10A (ATP10A Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
p6187
Plasmid#198158Purposebacterial expression of sifA::HaloTag fusion, mutant formDepositorInsertPsifA sifA delta331-336::L16::HaloTag::HA (sifA Salmonella enterica serovar Typhimurium)
UseTagsHaloTag-HAExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
MutaT7
Bacterial Strain#156460PurposeAn E. coli strain that expresses MutaT7 for mutagenizing genes downstream from a T7 promoter.DepositorBacterial ResistanceStreptomycinSpeciesRattus norvegicusAvailable sinceOct. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
VRK1_HUMAN_D0
Plasmid#79684PurposeThis plasmid encodes the kinase domain of VRK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertVRK1 (VRK1 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
sgGFP_3
Plasmid#78165PurposesgGFPDepositorInsertGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
SunTagng22aa
Plasmid#106438PurposeEncodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.DepositorInsertNOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-FerH-ffLuc2-eGFP
Plasmid#71393PurposeLentiviral vector of luciferase-eGFP fusion gene driven by FerH promoterDepositorInsertFerH-ffLuc2-eGFP
UseLentiviralTagsExpressionMammalianMutationPromoterFerHAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-10:LMNB1-mEGFP
Plasmid#87422PurposeHomology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1DepositorInsertLMNB1 Homology Arms with mEGFP-linker (LMNB1 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMarch 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEvolvR-enCas9-PolI3M-TBD
Plasmid#113077PurposeExpresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI3M-TBD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sema3g.a(L)-Fc-His
Plasmid#72151PurposeExpresses the Sema3G, isoform a protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema3g.a (Sema3g Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V1)
Plasmid#55216DepositorTypeEmpty backboneUseTagsSNAPf and TwinStrepIIExpressionInsectMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
mT-Sapphire-C1
Plasmid#54545PurposeLocalization: C1 Cloning Vector, Excitation: 399, Emission: 511DepositorTypeEmpty backboneUseTagsmT-SapphireExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRsetB-PercevalHR
Plasmid#49081PurposeBacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.DepositorInsertPercevalHR
UseTags7xHisExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-pDEST_TMEM192_BirA_Flag
Plasmid#227958PurposeBioID experimentDepositorInsertTMEM192 (TMEM192 Human)
UseTagsBirA-FLAGExpressionMammalianMutationR118G in BirAPromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOGS539_GLuc-ns
Plasmid#226719PurposePlasmid enabling yeast-mediated expression of Gaussia luciferase (GLuc)DepositorInsertGaussia Luciferase
UseTagsHA tagExpressionYeastMutationPromoterpTEF1Available sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only