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Plasmid#179321PurposeExpress mouse cholecystokinin 2 receptor (untagged) in mammalian cellsDepositorInsertmouse cholecystokinin2 receptor
UseTagsExpressionMammalianMutationstop codon after ORFPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-NucGFP
Plasmid#184706PurposeLentiviral transfer plasmid for expression of NucGFPDepositorInsertNucGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-3xGFP-tDeg
Plasmid#191371PurposeOverexpression of 3xGFP-tDeg in human cellsDepositorInsert3xGFP-tDeg
UseTags3xGFPExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-GFP-9S [rpoA]
Plasmid#176221PurposeExpression of GFP-9S [rpoA]DepositorInsertGFP-9S [rpoA]
UseSynthetic BiologyTagsHis-GFPExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCherry2-CD86-mEGFP
Plasmid#190748PurposeExpression of CD86 double tagged with mCherry2 (N-term) and mEGFP (C-term) in mammalian cells.DepositorInsertCD86 (CD86 Human)
UseTagsmCherry2 and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
mRFP-PA-GFP-RhoAQ63L
Plasmid#187282PurposeExpress pmRFP-PAGFP-RhoA Q63LDepositorInsertRhoA (RHOA Human)
UseTagsmRFP-PAGFPExpressionMammalianMutationRhoA Q63LPromoterCMV, T7Available sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-LuzGFPHis
Plasmid#188315PurposeExpression of Luz-eGFP via a T7 promoter in TXTLDepositorInsertLuz-GFP fusion
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-HisGFPLuz
Plasmid#188314PurposeExpression of eGFP-Luz via a T7 promoter in TXTLDepositorInsertGFP-Luz fusion
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpVasa-P662K-GFP
Plasmid#185591Purposeto see protein dynamicsDepositorInsertVasa
UseIn vitro transcription (mrna synthesis)TagsGFPExpressionMutationP662KPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpVasa-S83K-GFP
Plasmid#185588Purposeto see protein dynamicsDepositorInsertVasa-S83K
UseIn vitro transcription (mrna synthesis)TagsGFPExpressionMutationS83KPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpVasa-E441Q-GFP
Plasmid#185590Purposeto see protein dynamicsDepositorInsertVasa-E441Q
UseIn vitro transcription (mrna synthesis)TagsGFPExpressionMutationE441QPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
MiniSOG-SpAGS-GFP
Plasmid#185581Purposefor CALIDepositorInsertminiSOG-SpAGS
UseIn vitro transcription (mrna synthesis)TagsGFPExpressionMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpAGS-dGoLoco1-GFP
Plasmid#185577Purposefor overexpressionDepositorInsertAGS-dGoLoco1
UseIn vitro transcription (mrna synthesis)TagsGFPExpressionMutationdeletion of GoLoco motif #1 (aa473 DNFFEALSRFQSNR…PromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUAS-5-T7-TetO-GFP_ColE1
Plasmid#171660PurposeQUAS five base pairs upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli according to the Q and Tet systems. Contains the ColE1 origin of replication.DepositorInsertQUAS-5-T7-TetO-GFP ssra-T7 Stop
UseTagsssrA degradation tag (DAS+4)ExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUAS-0-T7-TetO-GFP_ColE1
Plasmid#171662PurposeQUAS directly upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli. Contains the ColE1 origin of replication.DepositorInsertQUAS-T7-TetO-GFP ssrA-T7 Stop
UseTagsssrA degradation tag (DAS+4)ExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NDUFA5-mVenus
Plasmid#185646PurposeNDUFA5 Complex I sub-unit with C-terminus mVenus tagDepositorInsertNDUFA5 (NDUFA5 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NDUFB6-mVenus
Plasmid#185652PurposeNDUFB6 Complex I sub-unit with C-terminus mVenus tagDepositorInsertNDUFB6 (NDUFB6 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NDUFS3-mVenus
Plasmid#185649PurposeNDUFS3 Complex I sub-unit with C-terminus mVenus tagDepositorInsertNDUFS3 (NDUFS3 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 shFmnl2-EGFP
Plasmid#187255PurposeLentiviral expression of shRNA targeting Fmnl2DepositorInsertShRNA for Fmnl2 (Fmnl2 Mouse)
UseLentiviralTagsEGFPExpressionMutationPromoterU6Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0-EGFP-HRasC20
Plasmid#187252PurposeLenticiral expression of EGFP-HRasC20DepositorInsertHRasC20 (HRAS Human)
UseLentiviralTagsEGFPExpressionMutationPromoter5' LTRAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only