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E3_pENTR_L3-L2_NN_G1397-DddA11'-N

Plasmid

#191614

Purpose

ptpTALECD_v2 vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_HD_G1397-DddA11'-C

Plasmid

#191603

Purpose

ptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11′), and a uracil glycosylase inhibitor

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_NG_G1397-DddA11'-C

Plasmid

#191604

Purpose

ptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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E1_pENTR_L1-L4_NI_G1397-DddA11'-C

Plasmid

#191605

Purpose

ptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
E1_pENTR_L1-L4_NN_G1397-DddA11'-C

Plasmid

#191606

Purpose

ptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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GOSR2(G144W)-mCherry

Plasmid

#209887

Purpose

Expresses human GOSR2 (GS27) G144W-mutant fused to mChery in mammalian cells

Depositor

Geert van den Bogaart

Insert

GOSR2 (GOSR2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Changed glycine 144 to tryptophan; this mutation …

Promoter

CMV

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGWBhis03-Citrine-NLS

Plasmid

#188558

Purpose

Binary vector for the expression of Citrine-NLS with MpIGPDm selective marker in Marchantia polymorpha (igpd mutants) .

Depositor

Yutaka Kodama

Article

Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

Insert

Citrine-NLS and MpIGPDm

Use

CRISPR; Entry vector

Tags

Expression

Mutation

Promoter

Available since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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AAV2/9-shMBVR-HO1-Fd-Fnr-CW3SL

Plasmid

#184003

Purpose

AAV production

Depositor

Vladislav Verkhusha, Dmitry Gerashchenko

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

shMBVR-HO1-Fd-Fnr-CW3SL

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-sgRNA-Target1 (MpRTN1)

Plasmid

#186291

Purpose

Gateway entry vector for sgRNA (target 1: MpRTN1). Transient expression of sgRNA (Target 1: MpRTN1) for MpRTN1 in plant cells

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

sgRNA-Target1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265N1

Plasmid

#173998

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-UNG for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-UNG

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

D10A

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ265O1

Plasmid

#173999

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-rXRCC1 for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

rAPOBEC1-zCas9(D10A)-rXRCC1

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

D10A

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_E2At(2nd)

Plasmid

#183085

Purpose

2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

E2A

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_O2At(2nd)

Plasmid

#183086

Purpose

2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

O2A

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2At(2nd)

Plasmid

#183087

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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DUX4_KBM pDonor221

Plasmid

#187802

Purpose

DUX4 with KBM (p. E414-L424 deletion) entry clone

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

DUX4-KBM (DUX4 Human)

Use

Tags

Expression

Mammalian

Mutation

p. E414-L424 deletion

Promoter

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pJC7

Plasmid

#177872

Purpose

Co-express in mammalian cells two target proteins, using an internal ribosome entry site and two multi-cloning sites.

Depositor

Roberto Dominguez

Article

Carman et al J Biol Chem. 2021 Oct;297(4):101154. doi

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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C232-E08: attB2r-mTFP1-attB3

Plasmid

#162904

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mTFP1 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Teal fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pZA52

Plasmid

#174765

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E/D481V with STOP codon

Use

Unspecified

Tags

Expression

Mutation

L17E/D481V + STOP codon

Promoter

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pZA50

Plasmid

#174763

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-D481V with STOP codon

Use

Unspecified

Tags

Expression

Mutation

D481V + STOP codon

Promoter

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pZA51

Plasmid

#174764

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E with STOP codon

Use

Unspecified

Tags

Expression

Mutation

L17E + STOP codon

Promoter

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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