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Plasmid#165886PurposeHygromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screeningDepositorInsertTransgenesis Ready Selection Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-Dusp6DM_S159/197A
Plasmid#67684Purpose3' entry clone with activated Dusp6 (S159/167A)DepositorInsertdusp6 (dusp6 Zebrafish)
UseTagsExpressionMutationS159A, S197APromoterAvailable sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
eDrTrkA
Plasmid#183996PurposeOptically controlled eDrTrkA expression in mammalian cellsDepositorInserteDrTrkA (NTRK1 Human)
UseTagsIg kappa secretory signal, mCherry, Deinococcus r…ExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEN-R2-mCsfGFP-L3
Plasmid#178602PurposeIt can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.DepositorInsertTandem fluorescent protein timers (tFTs)
UseEntry vector for gateway cloningTagsmCherry superfolder GFPExpressionMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR4_FLAG/HA_FUS_His6
Plasmid#26365DepositorInsertFUS (FUS Human)
UseEntry vectorTagsFLAG/HA and His6ExpressionMutationdelta 527 (stop codon)PromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBW313lux-hrpR
Plasmid#61436PurposepBWF2620 encoding rbs33-hrpR-ter (pBWF2620: pSB3K3 carrying BBa_F2620 (PTet-rbs34-luxR-ter))DepositorInsertF2620-rbs33-hrpR-ter
UseTagsExpressionBacterialMutationPromoterpluxAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132D2.0
Plasmid#99890PurposeGolden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBW300ParsR-Amp32T
Plasmid#78652PurposepSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-32hrpS-t-hrpL-30gfp-tDepositorInsertaraC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREG(R1-R3) #BGV141
Plasmid#48958PurposeRetroviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).DepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ256
Plasmid#124312PurposeGateway entry vector with PmCDA1 and pcoCas9D10A for C-T base editingDepositorInsertpcoCas9D10A-PmCDA1-UGI
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pre_IV_CS8_7569_v1
Plasmid#126865Purpose7886 bases long scaffold with Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 8, made by method IV, yields linear 7569 b long scaffolds after Zn incubationDepositorInsertIV_CS8_7569_v1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-p27(kip1)
Plasmid#50026PurposepENTR vector for human cyclin-dependent kinase inhibitor 1BDepositorInsertp27(kip1) (CDKN1B Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceDec. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCheck2 Dest (R1-R2) #BGV129
Plasmid#48955PurposeDestination dual luciferase reporter plasmid for checking effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway Entry vectors.DepositorTypeEmpty backboneUseLuciferase; Gateway cloning vectorTagsExpressionMutationPromoterSV40Available sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-SLC7A11 sh926R
Plasmid#118701Purposeentry clone for SLC7A11 insensitive to hairpin TRCN0000288926DepositorInsertSLC7A11 (SLC7A11 Human)
UseTagsExpressionMammalianMutationwt , SLC7A11 sh926RPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
KTK_001
Plasmid#180517PurposeEmpty backbone for creation of entry parts for the KTK, contains sfGFP dropoutDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(rhesus)
Plasmid#79033PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(rhesus)
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterpolyhedrinAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ERBB4 (R544W)
Plasmid#29531DepositorInsertERBB4 (ERBB4 Human)
UseTagsExpressionMammalianMutationchanged arginine 544 to tryptophanPromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
RAMA-bio-His
Plasmid#50737PurposeExpresses enzymatically monobiotinylated full length RAMA ectodomainDepositorInsertRAMA
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-M2-TLR2-V660N
Plasmid#12292DepositorInsertTLR2 (Tlr2 Mouse)
UseTagsFLAG (FLAG-M2)ExpressionMammalianMutationM82I, H202Y and V660N; signal peptide (aa1-23) re…PromoterAvailable sinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLJM60 FLAG NUFIP1 W40A
Plasmid#110951Purposeexpresses mammalian FLAG-NUFIP1 W40ADepositorInsertNUFIP1 W40A (NUFIP1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationchanged Tryptophan 40 to alaninePromoterCMVAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only