We narrowed to 9,738 results for: try

1. amilGFP chromoprotein

   Plasmid

   #117842

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses amilGFP chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, amilGFP
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRExT2A-CT-PmSc

   Plasmid

   #232549

   Purpose

   Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-PAC-3xTy

   Depositor

   Calvin Tiengwe

   Article

   Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

   Insert

   mScarlet
   

   Expression

   Bacterial

   Mutation

   None

   Available Since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223-VPS35

   Plasmid

   #21689

   Depositor

   Lynda Chin

   Article

   Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

   Insert

   Vacuolar Protein Sorting 35 homolog (VPS35 Human)
   

   Use

   Gateway entry clone

   Mutation

   last nucleotide of stop codon removed for c-termi…

   Available Since

   April 30, 2010

   Availability

   Academic Institutions and Nonprofits only

4. cjBlue chromoprotein

   Plasmid

   #117844

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses cjBlue chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, cjBlue
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMuLE Lenti Dest Neo

   Plasmid

   #62178

   Purpose

   MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses Neomycin cassette under the control of a PGK promoter. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Type

   Empty backbone

   Use

   Lentiviral; Mule gateway destination vector

   Expression

   Mammalian

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-GFP-WT1 +KTS N terminal HA

   Plasmid

   #217639

   Purpose

   Entry vector with WT1 +KTS mutant with N terminal HA for gateway cloning

   Depositor

   Constanze Bonifer

   Article

   Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

   Insert

   WT1 +KTS isoform (WT1 Human)
   

   Use

   Gateway cloning

   Tags

   HA

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-GFP-WT1 -KTS N terminal HA

   Plasmid

   #217641

   Purpose

   Entry vector with WT1 -KTS mutant with N terminal HA for gateway cloning

   Depositor

   Constanze Bonifer

   Article

   Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

   Insert

   WT1 -KTS isoform (WT1 Human)
   

   Use

   Gateway cloning

   Tags

   HA

   Mutation

   p.408_410delKTS

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAd MafA-I-nGFP

   Plasmid

   #19412

   Depositor

   Douglas Melton

   Article

   Zhou et al Nature. 2008 Oct 2;455(7213):627-32. doi

   Insert

   v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian) (Mafa Mouse)
   

   Use

   Adenoviral; Entry vector

   Tags

   GFP and IRES

   Available Since

   Nov. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pENTR-N90-beta-catenin

   Plasmid

   #31787

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   beta-catenin (CTNNB1 Human)
   

   Use

   Entry vector

   Tags

   Myc

   Mutation

   Deletion of aa 1-90- Constitutively Active

   Available Since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME V5-mTagBFP2 (JDW 830)

    Plasmid

    #224484

    Purpose

    Gateway compatible middle entry clone containing V5 tagged mTagBFP2 (Cytosolic blue fluorescent reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    V5-mTagBFP2
    

    Use

    Gateway cloning

    Available Since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. meffRed chromoprotein

    Plasmid

    #117836

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses meffRed chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, meffRed
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGTM_COV2_NSP5_C145A_001_SUMO

    Plasmid

    #192349

    Purpose

    Expresses SARS-CoV-2 3CLpro with an active site mutation (C154A) for the expression of the mature protease in an inactive form

    Depositor

    Gaetano Montelione

    Article

    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

    Insert

    SARS-COV-2 Mpro-C145A (NEWENTRY SARS-CoV-2)
    

    Tags

    6X-His-Sumo

    Expression

    Bacterial

    Mutation

    Cysteine 145 of the active site is substituted wi…

    Promoter

    T7 lac

    Available Since

    June 3, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. p5E TRE-3GV (JDW 1163)

    Plasmid

    #224476

    Purpose

    Gateway compatible 5' entry clone containing a 3rd generation Tet-On promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    TRE3GV promoter
    

    Use

    Gateway cloning

    Available Since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV2/9-CW3SL-mCherry-DrTrkB

    Plasmid

    #184002

    Purpose

    AAV production for expression of optically controlled eDrTrkB with N-terminal fusion of mCherry

    Depositor

    Vladislav Verkhusha, Dmitry Gerashchenko

    Article

    Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

    Insert

    eDrTrkB (NTRK2 Human)
    

    Use

    AAV

    Tags

    Ig kappa secretory signal, mCherry, Deinococcus r…

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME GFP-PCNA

    Plasmid

    #105977

    Purpose

    entry clone for gateway cloning, cell cycle marker proliferative cell nuclear antigen PCNA

    Depositor

    Caren Norden

    Article

    Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

    Insert

    PCNA (PCNA Human)
    

    Tags

    GFP

    Expression

    Bacterial

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2T-CAG-KKHSaCas9-BlastR

    Plasmid

    #107189

    Purpose

    Confers constitutive expression of KKH SaCas9.

    Depositor

    Richard Sherwood

    Article

    Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

    Insert

    KKH SaCas9 (NEWENTRY )
    

    Expression

    Bacterial

    Mutation

    E782K/N968K/R1015H

    Promoter

    Chicken ß-Actin

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. SynNotch TMD/Gal4-VP64 ICD (MTK 3b) (pAN1031)

    Plasmid

    #194222

    Purpose

    Mammalian Toolkit part 3b encoding SynNotch and GAL4-VP64 to be used in designing modular receptors

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    SynNotch-GAL4 DBD-VP64 AD
    

    Use

    Synthetic Biology

    Promoter

    None

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. dpYPQ230-D156R-CBE1

    Plasmid

    #195367

    Purpose

    dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN with 3xFLAG

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

    Insert

    hA3A/Y130F-XTEN with 3xFLAG-dLbCas12a-D156R-UGI
    

    Use

    CRISPR; Gateway compatible ha3a/y130f-xten plus 3…

    Tags

    3xflag

    Expression

    Plant

    Promoter

    ZmUBI1

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR

    Plasmid

    #136382

    Purpose

    CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion protein

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b (D570A)-TV-T2A-MCP-VPR
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D570A

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. p5E-hs_ICAM_full (JDW 1047)

    Plasmid

    #224479

    Purpose

    Gateway compatible 5' entry clone containing the Human ICAM2 promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    ICAM2 promoter
    

    Expression

    Mammalian

    Available Since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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