We narrowed to 9,771 results for: try

1. amilCP_Orange chromoprotein

   Plasmid

   #117850

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Orange chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, amilCP_Orange
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   64V,65G, BioBrick sites removed

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. spisPink chromoprotein

   Plasmid

   #117839

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses spisPink chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, spisPink
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-B/c

   Plasmid

   #227962

   Purpose

   Entry plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

   Depositor

   Alberto Carbonell

   Article

   Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   B/c
   

   Promoter

   None

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. asPink chromoprotein

   Plasmid

   #117838

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses asPink chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, asPink
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-BLM

   Plasmid

   #80070

   Purpose

   Expresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)

   Depositor

   Nathan Ellis

   Article

   Hu et al Hum Mol Genet. 2001 Jun 1;10(12):1287-98

   Insert

   BLM (BLM Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   If correct ID, the cDNA is from HeLa and has char…

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMuLE Lenti Dest eGFP

   Plasmid

   #62175

   Purpose

   MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses EGFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Type

   Empty backbone

   Use

   Lentiviral; Mule gateway destination vector

   Expression

   Mammalian

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMuLE Lenti Dest iRFP

   Plasmid

   #62176

   Purpose

   MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses iRFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Type

   Empty backbone

   Use

   Lentiviral; Mule gateway destination vector

   Expression

   Mammalian

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. eforRed chromoprotein

   Plasmid

   #117837

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses eforRed chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, eforRed
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Htr5b(3.7)-tTA-sv40

   Plasmid

   #184412

   Purpose

   AAV expression of tTA from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.

   Depositor

   Marylka Yoe Uusisaari

   Article

   Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

   Insert

   Mouse Htr5b gene promoter-tTA
   

   Use

   AAV

   Tags

   tTA

   Expression

   Mammalian

   Promoter

   Mouse Htr5b gene

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR-MBD1

    Plasmid

    #47057

    Purpose

    Expresses methyl-CpG binding domain protein 1

    Depositor

    Huda Zoghbi

    Article

    Sakai et al Sci Transl Med. 2011 Jun 8;3(86):86ra49.

    Insert

    Methyl-CpG Binding Domain Protein 1 (MBD1 Human)
    

    Use

    Gateway entry vector

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pSG4K5

    Plasmid

    #74492

    Purpose

    For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRExT2A-NT-PmNG

    Plasmid

    #232200

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mNeonGreen

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    mNeongreen
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXD71Cas10.0-Pct5.1-crRNA(AarI)

    Plasmid

    #191633

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer), entry plasmid for crRNA cloning

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    CymR
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. meffBlue chromoprotein

    Plasmid

    #117845

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses meffBlue chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, meffBlue
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME-H2B-mCerulean3-P2A-mKOFP2-CAAX

    Plasmid

    #75154

    Purpose

    Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    H2B-mCerulean3-P2A-mKOFP2-CAAX
    

    Use

    Middle entry vector for multisite gateway three f…

    Promoter

    n/a

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMCS-rybozyme-IRES-CAS9

    Plasmid

    #64668

    Purpose

    Plasmid encoding multiple cloning site, rybozyme and IRES-CAS9.

    Depositor

    Wataru Fujii

    Article

    Yoshioka et al Sci Rep. 2015 Dec 16;5:18341. doi: 10.10

    Inserts

    CAS9
    HDV ribozyme
    

    Use

    CRISPR

    Tags

    Flag, Internal ribosome entry site, and guide RNA…

    Expression

    Mammalian

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. p2T-CAG-SpCas9-BlastR

    Plasmid

    #107190

    Purpose

    Confers constitutive expression of SpCas9

    Depositor

    Richard Sherwood

    Article

    Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

    Insert

    SpCas9 (NEWENTRY )
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Bacterial and Mammalian

    Promoter

    Chicken ß-actin

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221_SLC6A3

    Plasmid

    #132160

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Sijben et al Sci Rep. 2021 Jan 14;11(1):1312. doi: 10

    Insert

    SLC6A3 (SLC6A3 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. cjBlue chromoprotein

    Plasmid

    #117844

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses cjBlue chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, cjBlue
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223-VPS35

    Plasmid

    #21689

    Depositor

    Lynda Chin

    Article

    Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

    Insert

    Vacuolar Protein Sorting 35 homolog (VPS35 Human)
    

    Use

    Gateway entry clone

    Mutation

    last nucleotide of stop codon removed for c-termi…

    Available Since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only