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Plasmid#86013PurposeExpression of dengue virus 3 NS5 proteinDepositorInsertDENV3-NS5FL
UseTagshexa-histidine tagExpressionBacterialMutationamino acid residues 6 to 895 of DENV3 NS5PromoterT7Available sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-AR
Plasmid#28235PurposeMammalian expression of Androgen receptor fused to EGFPDepositorInsertAndrogen receptor (AR Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme WT
Plasmid#18110DepositorInsertT4 Lysozyme (e Bacteriophage T4)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-HIV RT-6xHis-RBS-prot
Plasmid#159149Purposeexpresses His-tagged HIV reverse transcriptase and untagged HIV proteaseDepositorInsertsHuman immunodeficiency virus 1 (HIV-1) reverse transcriptase
Human immunodeficiency virus 1 (HIV-1) protease
UseTagsHisExpressionBacterialMutationHIV-1 group M/subtype B – BH10 strainPromoterAvailable sinceSept. 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-FLEX-EGFPL10a
Plasmid#98747PurposeCre-dependently expresses the fusion protein EGFPL10aDepositorHas ServiceAAV Retrograde and AAV5InsertEGFPL10a
UseAAV and Cre/LoxTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiCas9n(D10A)-Blast
Plasmid#63593PurposeExpresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.DepositorInsertsCas9
Blasticidin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationD10A, nickase mutantPromoterEFS-NSAvailable sinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-puro-cOVA
Plasmid#135073PurposeExpresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistanceDepositorInsertcOVA (OVAL Chicken)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF3365
Plasmid#144841PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFOXA1 (FOXA1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_LASV_GPC
Plasmid#207287PurposeMammalian expression of Lassa virus GPC proteinDepositorInsertLASV_GPC
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-luciferase
Plasmid#55764Purposeluciferase reporter for in vivo bioluminescence imagingDepositorInsertCMV enhancer/chicken beta actin promoter
UseTagsExpressionMammalianMutationPromoterCMV enhancer/chicken beta actin promoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamKIIa-jGCaMP8m-WPRE
Plasmid#176751PurposeAAV-mediated expression of GCaMP8m under the CamKIIa promoterDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8m
UseAAVTags6xHisExpressionMammalianMutationPromoterCamKIIalphaAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-BlaM-Vpr
Plasmid#21950DepositorInsertBlaM-Vpr (vpr Fusion of BlaM and HIV-1 Vpr)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
PJQ49
Plasmid#134657PurposeContains Promoter 3 with bsa1 cut sites for inserting sgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAnderson BBa_J23115Available sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IP GFP-NBR1
Plasmid#38283DepositorInsertNext to BRCA1 gene 1 protein (NBR1 Human)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJ404-DogCatcher-sfGFP
Plasmid#171930PurposeExpress DogCatcher-superfolder GFP protein in bacterial cytoplasmDepositorInsertDogCatcher-sfGFP
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT5Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Luc-T20
Plasmid#177941PurposeTransient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.DepositorInsertLuc2 (ORF1ab )
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
PJQ47
Plasmid#134646PurposeContains Promoter 1 with bsa1 cut sites for inserting sgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAnderson BBa_J23117Available sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
PJQ48
Plasmid#134647PurposeContains Promoter 2 with bsa1 cut sites for inserting sgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAnderson BBa_J23114Available sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_WT POLH
Plasmid#201671PurposeExpression of WT DNA polymerase eta with an N-terminal HA tag in mammalian cellsDepositorInsertPolymerase eta (POLH Human)
UseTagsHAExpressionMammalianMutationCoding sequence has been optimised for expression…PromoterCMVAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTUB1:OsTIR1-3FLAG, SAG1:CAT
Plasmid#87258PurposeTIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.DepositorInsertsTIR1
CAT
UseToxoplasma expressionTags3FLAGExpressionMutationCodon optimized for Toxoplasma gondii expressionPromoterTgSAG1 361 bp and TgTUB1 2717 bpAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only