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Plasmid#118707Purposereporter plasmid, HIF-responsive promoter (3xHRE) mutant- fused to firefly luciferaseDepositorInsertHREmutant-Luc
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQCascade
Plasmid#140622PurposeExpresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and CRISPR RNADepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX ShcD-SH2
Plasmid#46488DepositorInsertShcD (SHC4 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 512-623PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQE32-pr-mEosFP (mEosFP-V69T)
Plasmid#99213PurposeBacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).DepositorInsertpr-mEosFP
UseTagsExpressionBacterialMutationmonomeric variant with mutation V69T for primed c…PromoterT5Available sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.4
Plasmid#111057PurposeExpresses dLight1.4 in mammalian cellsDepositorInsertdLight1.4
UseTagsFlag tagExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Caveolin-N-10
Plasmid#54026PurposeLocalization: Membrane, Excitation: 487, Emission: 509DepositorInsertCaveolin (CAV1 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS
Plasmid#154774Purposeplasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma PylRS for ochre suppression in transient or stable, piggybac-mediated, integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAGExpressionMammalianMutationin PylT: UUA anticodon and G10A, G14U, G16U, C20U…PromoterEF1Available sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
MoFGFR1c-ires neo
Plasmid#86490Purposemamalian cell expression of FGFR1cDepositorInsertFgfrR1 IIIc 15D8 (Fgfr1 Mouse)
UseTagsExpressionMammalianMutationPromoterMoloney murine leukemia virus LTRAvailable sinceFeb. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE-60 Grx1-roGFP2-His
Plasmid#64799PurposeBacterial expression of His-tagged Grx1-roGFP2DepositorInsertGlutaredoxin-1 (GLRX Human)
UseTagsHis and roGFP2ExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-LaminB1-10
Plasmid#58020PurposeLocalization: Nuclear Envelope, Excitation: 555, Emission: 584DepositorInsertLaminB1 (LMNB1 Human)
UseTagsmTagRFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
MSP1673
Plasmid#65769PurposeBacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNADepositorInsertmammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
UseCRISPRTagsNLSExpressionBacterialMutationPromoterT7 (x2)Available sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKD072
Plasmid#136472PurposeSSB-C-term-GFP fusion expression plasmidDepositorInsertSSB-C-term-GFP (ssb E. coli)
UseTagssuperfolder GFPExpressionBacterialMutationsuperfolder GFP attached to C-terminus at Phe 178PromoterT7 promoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Sox2-CreERT2
Plasmid#135618PurposeDonor vector for genomic targeting of a P2A-CreERT2 cassette to the 3' end of the mouse Sox2 locusDepositorInsertP2A-CreERT2 flanked by Sox2 homology arms (Sox2 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsP2A-CreERT2ExpressionMutationPromoterAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-Tollip M240A,F241A
Plasmid#178051PurposeGFP fused to human Tollip cDNA carrying mutations in the CUE domain. Localisation and expression in mammalian cells.DepositorInsertToll-Interacting Protein (TOLLIP Human)
UseTagsGFPExpressionMammalianMutationChanged Methionine 240 to Alanine and changed Phe…PromoterCMVAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE
Plasmid#138146PurposeExpresses PspCas13b in mammalian cells, localized in the cytoplasm. For cloning guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang acac. R overhang caacDepositorInsertPspCas13b
UseLentiviralTagsNESExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
IMPT-10316:GABR1
Plasmid#157850PurposeHA-TEV-VFT-TMD-CC-PreX-eGFP in pFastBac1 for Sf9 expressionDepositorInsertGABR1 (GABBR1 Human)
UseTagsExpressionInsectMutationPromoterPolyhedrinAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6-Slc1a3 sgRNA; EF1a-dCas9-KRAB-GFP
Plasmid#194283PurposeEF1a-dCas9-KRAB-GFP with Slc1a3 sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterEF1aAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
U6-NT sgRNA; EF1a-dCas9-KRAB-GFP
Plasmid#194284PurposeEF1a-dCas9-KRAB-GFP with nontarget sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterEF1aAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV-NLS-ISAam1-NLS-3xHA-bGHpA;U6-BbsI-reRNA
Plasmid#205316PurposeAll-in-one AAV vector expressing ISAam1 TnpB and its reRNADepositorInserthumanized ISAam1 TnpB
UseAAVTags3 x HAExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only