-
Plasmid#194250PurposeHuman RNAP1 promoter region driving firefly luciferase expression from IRES. Constructed by Ghoshal, et al. J Biol Chem (2004). Please cite this original manuscript!DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterhuman rDNA promoter and beginning of 5' ETS …Available sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-let-7 WT
Plasmid#78260PurposepsiCHECK2 dual luciferase reporter harboring a fully complementary let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase geneDepositorInsertfully complimentary let-7 target site
UseLuciferase; Microrna activityTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
p CIneo-RL-Let7-3xBulgeB-mut
Plasmid#115369PurposeExpression vector to produce humanized Renilla luciferase with three mutated (seed-sequence mutations) partially complementary binding sites for human let-7 miRNA in the 3'UTRDepositorInsertRenilla Luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
miR-34aM2
Plasmid#50828Purposeluciferase reporter for human miR-34aDepositorInsertmiR-34A promoter M2 (MIR34A Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationsecond NF-kB binding site mutated (-511)PromotermiR34aAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
miR-34aM53
Plasmid#50830Purposeluciferase reporter for human miR-34aDepositorInsertmiR-34A promoter M53 (MIR34A Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationp53 binding site mutated (-39)PromotermiR34aAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_LuxSit_i_mTagBFP2
Plasmid#194300PurposeMammalian expression of the de novo luciferase, LuxSit-iDepositorInsertLuxSit-i
UseTagsmTagBFP2ExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-let-7 MT
Plasmid#78261PurposepsiCHECK2 dual luciferase reporter harboring a mutated let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase geneDepositorInsertmutated let-7 target site
UseLuciferase; Microrna activityTagsExpressionMammalianMutationnucleotides 2-5 and 10-11 changed from CTCC to GA…PromoterAvailable sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:GLuc:bgH
Plasmid#135294PurposeTranscriptional unit for constitutive expression of GLuc in mammalian cellsDepositorInsertCMV:GLuc:bGH
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSKC12 - DelQ
Plasmid#110493PurposeMonitor the expression of Luciferase driven by a mutated NRAS 5'-UTR.DepositorInsertNRAS 5'- DelQ (bp 30-254) (NRAS Human)
UseLuciferaseTagsFirefly luciferaseExpressionMutationDeletion of the first 29 base pairs of the NRAS 5…PromoterT7Available sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-miR-34 WT
Plasmid#78258PurposepsiCHECK2 dual luciferase reporter harboring a fully complementary miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase geneDepositorInsertfully complimentary miR-34 target site
UseLuciferase; Microrna activityTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-miR-34 MT
Plasmid#78259PurposepsiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase geneDepositorInsertmutated miR-34 target site
UseLuciferase; Microrna activityTagsExpressionMammalianMutationnucleotides 2-5 and 10-11 changed from CCGT to GG…PromoterAvailable sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFRTpsiCHECK
Plasmid#154454PurposeExpression of renilla luciferase and firefly luciferaseDepositorInsertRenilla luciferase, firefly luciferase
UseTagsExpressionMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIND-CLucZ
Plasmid#53224PurposeInducible expression of Cypridina luciferase and shRNAmir of interestDepositorInsertsCypridina luciferase
non-silencing shRNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
373_MLH1 in pmiRGlo
Plasmid#78133Purposeluciferase reporter for miRNA activityDepositorInsertMLH1 (MLH1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation3'UTR containing miRNA binding sitesPromoterPGKAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
PAK4-FL-3'UTR
Plasmid#113088PurposeLuciferase reporter containing PAK4- FL-3'UTRDepositorInsertp21 Activated kinase 4 (PAK4 Human)
UseLuciferaseTagsExpressionMammalianMutationnonePromoterPGKAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
miR-34aM3
Plasmid#50829Purposeluciferase reporter for human miR-34aDepositorInsertmiR-34A promoter M3 (MIR34A Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationthird NF-kB binding site mutated (-149)PromotermiR34aAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
miR-34aM1
Plasmid#50827Purposeluciferase reporter for human miR-34aDepositorInsertmiR-34A promoter M1 (MIR34A Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationfirst NF-kB binding site mutated, (-539)PromotermiR34aAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pL106P-LUC-ENTR1A(w451-1)
Plasmid#17455PurposeGateway entry vector, Degron N-term LuciferaseDepositorInsertFKBP-Luciferase fusion (MTOR firefly)
UseLuciferase; Entry vectorTagsFKBP12DDExpressionMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
1kb Zfp521 promoter reporter
Plasmid#104188Purposeluciferase reporter to assay Zfp521 promoter activationDepositorInsertZfp521 promoter - 1 Kb (Zfp521 Mouse)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoternoneAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMath3-luc
Plasmid#51312PurposeLuciferase reporter driven by the Math3 promoterDepositorInsertMath3 promoter (-2.8k- +196) (Neurod4 Mouse)
UseLuciferaseTagsluciferaseExpressionMutationPromoterAvailable sinceMarch 7, 2014AvailabilityAcademic Institutions and Nonprofits only