PAK4-FL-3'UTR
(Plasmid
#113088)
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PurposeLuciferase reporter containing PAK4- FL-3'UTR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113088 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmirGLO Dual-Luciferase
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 7350
- Total vector size (bp) 8140
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namep21 Activated kinase 4
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Alt namePAK4
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SpeciesH. sapiens (human)
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Insert Size (bp)790
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Mutationnone
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GenBank IDNM_001014834.2 NM_001014834.2
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Entrez GenePAK4
- Promoter PGK
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Dra I (not destroyed)
- 3′ cloning site Sac I (not destroyed)
- 5′ sequencing primer ACACGGTAAAACCATGAC
- 3′ sequencing primer GTCCAAACTCATCAATGTA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PAK4-FL-3'UTR was a gift from James Donahue (Addgene plasmid # 113088 ; http://n2t.net/addgene:113088 ; RRID:Addgene_113088) -
For your References section:
MiR-199a-3p decreases esophageal cancer cell proliferation by targeting p21 activated kinase 4. Phatak P, Burrows WM, Chesnick IE, Tulapurkar ME, Rao JN, Turner DJ, Hamburger AW, Wang JY, Donahue JM. Oncotarget. 2018 Jun 19;9(47):28391-28407. doi: 10.18632/oncotarget.25375. eCollection 2018 Jun 19. 10.18632/oncotarget.25375 PubMed 29983868