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Plasmid#92144PurposeFor transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locusDepositorInsertspCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG::Nucbow
Plasmid#158996PurposeMulticolor nuclear-restricted labeling with a genome-integrating piggyBac transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)DepositorInsertNucbow
UseTagsAll FPs are fused to H2BExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Flag-WTAP
Plasmid#53741PurposeFor expression of WTAP (Wilms tumor 1 associated protein) in human cellsDepositorInsertWilms tumor 1 associated protein (WTAP Human)
UseTagsFLAG tagExpressionMammalianMutationPromoterT7Available sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-cytoEKAR4
Plasmid#174438PurposeCytosol-targeted EKAR4.DepositorInsertcytoEKAR4
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pKM7
Plasmid#154164PurposemCherry expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.DepositorInsertmCherry
UseTagsExpressionInsectMutationPromoterIE1 promoter and hr5 enhancer from Autographa cal…Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K27
Plasmid#22902DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK27 only, all other lysines mutated to argininesPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen
Plasmid#182387PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen fusionDepositorInsertVNp-mNeongreen-His
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K11
Plasmid#22901DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK11 only, all other lysines mutated to argininesPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMAH-POLY-eRF1(E55D)
Plasmid#157925PurposeEncodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cellsDepositorInsertPOLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K6
Plasmid#22900DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK6 only, all other lysines mutated to argininesPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo-FLAG/HA Ago2 corrected
Plasmid#10822DepositorInsertArgonaute 2 (AGO2 Human)
UseTagsFLAG and HAExpressionMammalianMutation3 silent mutations in orfPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo-FLAG/HA Ago1
Plasmid#10820DepositorInsertArgonaute 1 (AGO1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-N-TEV-Twin-Strep
Plasmid#202526PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cellDepositorTypeEmpty backboneUseTagsTEV cleavable site and Twin-strep-tagExpressionMammalianMutationPromoterCAGAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRE-KRAB-dCas9-IRES-GFP
Plasmid#85556Purpose2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFPDepositorInsertKRAB-dCas9-IRES-GFP
UseCRISPR and LentiviralTags2x NLS, HA Tag, and KRAB domainExpressionMammalianMutationPromoterTRE3GAvailable sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 SA-2A-puro-pA donor
Plasmid#22075PurposeAAV zinc finger donor vector for genomic targetingDepositorInsertSA-Puro
UseZfn donorTagsExpressionMutationPromoterAvailable sinceOct. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-PTuner DD-linker-M.EcoGII
Plasmid#122082PurposeExpress M.EcoGII with detabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII
UseRetroviralTagsV5 tag on Nter of M.EcoGIIExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSJ1256 (pFA6-link-yGFP1-10-CaURA3MX)
Plasmid#86419PurposeC-terminal PCR tagging module for yeast codon optimized split GFP1-10DepositorInsertGFP1-10
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28-SaCas9
Plasmid#178901PurposeSaCas9 (Cas9 from Staphylococcus aureus)DepositorInsertSaCas9
UseTagsHis6-Tag, bipartite nuclear localization signal f…ExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -