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Plasmid#204634PurposeColE1 ori, KanR, TetR, Tet promoter with a codon optimized pbTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.DepositorInsertHis-SUMO-TEV-PbTTA
UseSynthetic BiologyTagsHis Tag; SUMO Tag; TEV Protease SiteExpressionBacterialMutationPromoterPLtetO-1 promoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NUP188
Plasmid#159893PurposeExpresses NUP188 in mammalian cellsDepositorInsertNUP188 (NUP188 Human)
UseTagsEGFPExpressionMammalianMutationTo avoid NotI site, first three amino acids are d…PromoterCMVAvailable sinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension
Plasmid#176542PurposeBacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locusDepositorInsertsMBP tag
TnpB
UseTags3' extension derived from endogenous locus a…ExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGRG36-Kn-PA1-GFP
Plasmid#79088PurposepGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)DepositorInsertGFP
UseBacterial chromosome integration and expression o…TagsExpressionBacterialMutationPromoterPA1Available sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX458-GFP-TRIM28
Plasmid#185010PurposeEncodes gRNA for mouse TRIM28 along with Cas9 with 2A-EGFPDepositorInsertTRIM28 sgRNA (Trim28 Mouse)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMIR-21 Reporter
Plasmid#71870PurposeMammalian expression vector for the analysis of miR-21 activityDepositorInsertReverse complementary sequence of miR-21
UseTagsfirefly luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEA01
Plasmid#170766PurposeUtilisation of pSAM2 excision tool in Amycolatopsis sppDepositorInsertsxis, int
oriT
pac
UseTagsExpressionBacterialMutationPromotertrcpAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-Blasticidin-hOCT4 WT
Plasmid#198764PurposeMammalian expression of hOCT4DepositorInserthOCT4 WT (POU5F1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS0781 pcDNA3.1(+) CMV-SV40 NLS-AwaIscB-nucleoplasmin NLS-3xHA
Plasmid#176538PurposeMammalian expression of nuclear localized AwaIscBDepositorInsertAwaIscB
UseTagsNucleoplasmin NLS-3xHA and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS1801
Plasmid#205966PurposeJAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promotersDepositorInsertCas8-HNH proteins and CRISPR array
UseTagsExpressionBacterialMutationPromoterLac (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1232
Plasmid#205969PurposeMWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promotersDepositorInsertCas5-HNH proteins and CRISPR array
UseTagsExpressionBacterialMutationPromoterLac (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC4-RhE-FRB-Fis1
Plasmid#68056Purposeexpress Fis1 tail-anchored FRB for heterodimerization with FKBP in mammalian cellsDepositorInsertFis1 (FIS1 Human)
UseTagsFRBExpressionMammalianMutationFis1 tail (aa 93-152)PromoterAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET19-M(BRCT)TdTwt
Plasmid#207459PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domainDepositorInsertM(BRCT)TdTwt (Dntt Mouse)
UseTags10xHis-MBPExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hPPP3CB-FLAG
Plasmid#179135PurposeExpression vector of human protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.DepositorInsertprotein phosphatase 3, catalytic subunit, beta isoform (PPP3CB Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-RUNX2(WT)-IDR-mCherry
Plasmid#145286PurposeBacterial Expression of 6xHis RUNX2(WT) IDR mCherry Fusion ProteinDepositorInsertRUNX2(WT)-IDR (RUNX2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5 FLAG wildtype, catalytic dead lipin 1
Plasmid#32006DepositorInsertLipin1 (Lpin1 Mouse)
UseTagsFLAGExpressionMammalianMutationD712E and D714EPromoterCMV, SP6Available sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPv2_AAVS1
Plasmid#184487PurposeLentivirus all in one CRISPR vector targeting human AAVS1 geneDepositorInsertAAVS1 (AAVS1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-NeoR_dCas9-Sniper-KRAB_sgSTK3i_#2 (NeoR)
Plasmid#209775PurposeCRISPRi for STK3DepositorInsertdCas9-Sniper-KRAB, sgSTK3i (STK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Tet2 promoter F
Plasmid#63879PurposeLuciferase reporter plasmid used to study transcriptional control of murine Tet2DepositorInsertTet2 Promoter fragment F
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
PLEX_313 SLC31A1
Plasmid#184493PurposeLentivirus expression for SLC31A1 geneDepositorInsertSLC31A1 (SLC31A1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only