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Plasmid#183972PurposeEnhanced RNAi clone for C. elegans knockdown of non-codon-optimized GFPDepositorInsertGFP (non-codon-optimized)
UseRNAiTagsExpressionWormMutationPromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-NeoCOVGT3-d2EGFP
Plasmid#201446PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT3_d2EGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKozak_mTagBFP2-T2A-sGFP11x7_synCoTC
Plasmid#199495PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2-T2A-sGFP11x7
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA_0_mTagBFP2-T2A-sGFP11x7_synCoTC
Plasmid#199478PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2-T2A-sGFP11x7
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA_1_mTagBFP2-T2A-sGFP11x7_synCoTC
Plasmid#199479PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2-T2A-sGFP11x7
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA_2_mTagBFP2-T2A-sGFP11x7_synCoTC
Plasmid#199480PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2-T2A-sGFP11x7
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Lyn11C32S-PIP4K2A
Plasmid#202748Purposeweakly plasma membrane targeted fluorescent protein-tagged enzymeDepositorInsertEGFP:LYN (1-11) (C3S):PIP4K2A (PIP4K2A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUt-mNF-GFP
Plasmid#199220PurposeLPUtopia-7 matching RMCE donor plasmid with GFP Negative-Feedback circuit (mNF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.DepositorInserthTetR::P2A::EGFP
UseTagsEGFPExpressionMammalianMutationPromoterCMV D2ir promoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUt-mPF-GFP
Plasmid#199222PurposeLPUtopia-7 matching RMCE donor plasmid with GFP Positive-Feedback circuit (mPF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.DepositorInsertrtTA-Advanced::P2A::EGFP
UseTagsEGFPExpressionMammalianMutationPromotertight TRE promoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eGFP-mPlum-HA
Plasmid#200009PurposeDonor vector with double fluorescence system for targeted integration using CRISPR/Cas9. Expresses 5´HA--eGFP&PuroR--3´HA--mPlum. Distinguish targeted from random integration by fluorescence.DepositorInserteGFP, mPlum
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#1
Plasmid#197424Purposeπ-element knock-in into exon 5 of MAPRE1DepositorInsertMAPRE1 gRNA #1 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#2
Plasmid#197425Purposeπ-element knock-in into exon 5 of MAPRE1DepositorInsertMAPRE1 gRNA #2 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#3
Plasmid#197426Purposeπ-element knock-in into exon 5 of MAPRE1DepositorInsertMAPRE1 gRNA #3 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-π-EB1_EF1α-EGFP
Plasmid#197430PurposeHomology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5DepositorInsertEF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
UseCRISPR and Synthetic BiologyTagsEGFP, GCN4 leucine zipper, LOV2, and Zdk1ExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PGK-EGFP-FKBPF36V
Plasmid#199765Purposeplasmid for targeting dTAG into the AAVS1 locusDepositorInsertGFP-dTAG
UseTagsGFP and dTAGExpressionMammalian and WormMutationPromoterhPGKAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 H237A-EGFP
Plasmid#201425PurposeBacterial protein expression of Dcp2 H237ADepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsEGFP, TEV, 6xHis and MBP, TEVExpressionBacterialMutationH237APromotertacAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
MCS:GFPnls-T2a-ER-Cre-ER
Plasmid#200106PurposeVector for LFNGDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
LFNG:GFPnls-T2a-ER-Cre-ER
Plasmid#200107DepositorInsertLFNG:GFPnls-T2A-ER-Cre-ER
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dhh1 25-425-EGFP
Plasmid#201430PurposeBacterial protein expression of Dhh1 25-425DepositorInsertDhh1 (DHH1 Budding Yeast)
UseTagsEGFP, TEV, 6xHis and MBP, TEVExpressionBacterialMutationaa 25-425 onlyPromotertacAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dcp1 H206A-EGFP
Plasmid#201424PurposeBacterial protein expression of Dcp1 H206ADepositorInsertDcp1 (DCP1 Budding Yeast)
UseTagsEGFP, TEV, 6xHis and MBP, TEVExpressionBacterialMutationH206APromotertacAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only