pUC19-π-EB1_EF1α-EGFP
(Plasmid #197430)

• Purpose: Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5
• Depositing Lab: Torsten Wittmann
• Publication: Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.7554/eLife.84143.

Backbone

• Vector backbone: pUC19
• Backbone size w/o insert (bp): 2681
• Total vector size (bp): 9025
• Vector type: CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site
• Alt name: EB1
• Species: H. sapiens (human)
• Insert Size (bp): 6344
• GenBank ID: NM_012325.2
• Entrez Gene: MAPRE1 (a.k.a. EB1)
• Tags / Fusion Proteins:
  • GCN4 leucine zipper
  • LOV2
  • EGFP
  • Zdk1

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13 forward
• 3′ sequencing primer: M13 reverse

Resource Information

• Supplemental Documents:
  • pUC19-piEB1-EGFP-knock-in, HDR plasmid, EF1alphapromoter.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.09.22.509085v1 for bioRxiv preprint.