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Viral Prep#109048-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST (#109048). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST plasmid DNA. Cre-dependent expression of the anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites, translated from an internal ribosomal entry site (IRES) following mRuby3 expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsmRuby3Available sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only
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pmScarlet-i_Giantin_C1
Plasmid#85050PurposeIn vivo visualization of the Golgi apparatus (can be used for colocalization studies)DepositorInsertGiantin (GOLGB1 Human)
UseTagsExpressionMammalianMutationaa 3131-3259PromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-BrCas12b-RFND
Plasmid#195339PurposeExpresses Engineered BrCas12b in BacteriaDepositorInsertBrCas12b
UseCRISPR and Synthetic BiologyTags6xHis and 6xHis, MBP, TEV cleavage siteExpressionBacterialMutationR160E, F208W, N524V, D868VPromoterT7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE38
Plasmid#90378PurposeRELB - NFkB gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterMAFAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVax1-hVEGF165
Plasmid#74466Purposeexpression of hVEGF165 in mammalian cellsDepositorInsertvascular endothelial growth factor 165 (VEGFA Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRK669
Plasmid#71313PurposeHelper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.DepositorTypeEmpty backboneUseE. coli-gram positive bacteria shuttle vectorTagsExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
negMaLionR
Plasmid#113909Purposenegative control for Red ATP sensorDepositorInsertnegMaLionR
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
MCS-3_Endofin_LgBiT
Plasmid#182574PurposeThis plasmid can be used to monitor receptor internalization in real time living cells using bioluminescence as a readout.DepositorInsertzinc finger FYVE-type (ZFYVE16 Human)
UseTagsLgBiT (Large BiT)ExpressionMammalianMutationFYVE domain Q739-K806PromoterHerpes simplex virus (HSV)Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
LLP791_PB_dCas9-SSSavi_BirA
Plasmid#211786PurposePiggyBac plasmid with dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), and BirA, with Hygro selectionDepositorInsertdCas9, Spy, Snoop, aGCN4, AviTags
UseTags3xHAExpressionMammalianMutationdCas9 D10A and H840APromoterpCAGG abd pEF1aAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT sfGPF 150TAG
Plasmid#154766Purposeplasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAG in GFP reporter, U25C in PylTPromoterEF1Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_alphaTubulin_C1
Plasmid#85045PurposeIn vivo visualization of microtubules (can be used for colocalization studies)DepositorInsertAlpha-Tubulin (TUBA1B Human)
UseTagsmScarletExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Myc3-CAND1
Plasmid#19948DepositorInsertCAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)
UseTagsmyc3ExpressionMammalianMutationPromoterAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TLR9-YFP
Plasmid#13642DepositorInsertToll-Like Receptor 9 (TLR9 Human)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceJune 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLEM415-ldhL-mRFP1
Plasmid#99842PurposeExpression of RFP in LactobacillusDepositorInsertmonomeric red fluorescent protein
UseTagsFLAGExpressionBacterialMutationPromoterldhlAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
HDAC8 Flag
Plasmid#13825DepositorInsertHDAC8 (HDAC8 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
VCP(R155H)-EGFP
Plasmid#23972DepositorInsertValosin containing protein (VCP Human)
UseTags6xHis, EGFP, and HAExpressionMammalianMutationR155HPromoterAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NES-jRCaMP1b
Plasmid#63136PurposeRed fluorescent calcium sensor proteinDepositorInsertNES-jRCaMP1b
UseTagsnuclear export signalExpressionMammalianMutationPromoterCMVAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
CaMKAR
Plasmid#205315PurposeFluorescent Reporter for CaMKII ActivityDepositorInsertCaMKAR
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMega-MaPylRS
Plasmid#200225PurposeThe plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.DepositorInsertsPyrrolysyl-tRNA synthetase
pyrrolysyl-tRNA
UseTagsnoneExpressionBacterialMutationnonePromoterproK-lacO and tacIAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-ER-LAR-GECO1
Plasmid#61244PurposeExpresses LAR-GECO1 in the endoplasmic reticulum in mammalian cellsDepositorInsertLAR-GECO1
UseTagsER-retention sequence: KDEL and ER-targeting sequ…ExpressionMammalianMutationSubstitutions relative to R-GECO1: V51W/I113V/N35…PromoterCMVAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only