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Plasmid#187259PurposeLentiviral expression of myosin regulatory light chain-AA mutantDepositorInsertMRLC1 (MYL9 Human)
UseLentiviralTagsEGFPExpressionMutationT18A,S19APromoter5' LTRAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-TJP1
Plasmid#227300PurposeDonor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299)DepositorInsertTJP1 Homology Arms flanking a mStayGold Tag (TJP1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
2_T7-mScarlet3-KRas
Plasmid#225929PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras membrane localisation signalDepositorInsertmScarlet3-KRas
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
5_T7-GAP43-mScarlet3
Plasmid#225932PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the GAP43 membrane localisation signalDepositorInsertGAP43-mScarlet3
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-TJP1
Plasmid#227299PurposeExpresses SpCas9 and a sgRNA targeting the N-terminus of TJP1 for knock-in.DepositorInsertsgRNA Targeting N-terminus of TJP1 (TJP1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBM4
Plasmid#225291PurposeE. coli-Campylobacter shuttle vector; TetRDepositorInsertTet(O) and its putative promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-flex-iGABASnFR2(no bind)-WPRE
Plasmid#218882PurposeAAV-mediated expression of improved GABA sensor control (no change in fluorescence)DepositorInsertiGABASnFR2(no bind)
UseAAV and Cre/LoxTagsExpressionMammalianMutationS99A F102G R168PPromoterCAGAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-flex-iGABASnFR2(no bind)-WPRE
Plasmid#218879PurposeAAV-mediated expression of improved GABA sensor control (no change in fluorescence)DepositorHas ServiceAAV1 and AAV5InsertiGABASnFR2(no bind)
UseAAV and Cre/LoxTagsExpressionMammalianMutationS99A F102G R168PPromoterSynapsinAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF1035
Plasmid#143746PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertWT1 (WT1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2903
Plasmid#143659PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertDACH1 (DACH1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1038
Plasmid#144075PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertWT1 (WT1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1570
Plasmid#144105PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF680 (ZNF680 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPARA1
Plasmid#200416PurposeGolden Gate assembly of expression constructs containing pBAD promoter (carbenicillin resistance)DepositorArticleTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterpBADAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRep
Plasmid#199599PurposeExpresses AAV2 rep gene; accessory plasmid for the pCMV-Cap SSM library.DepositorInsertAAV2 rep
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2511
Plasmid#195625PurposeComplementation of MlaA(WT)DepositorInsertMlaA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+7R+12D
Plasmid#178678PurposeBacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD+7R+12D (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationseven Arg residues added, twelve Glu residues add…PromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL1994
Plasmid#139898PurposeExpression plasmid for LetB PLL2 deletion (without TM region)DepositorInsertLetB
UseTagsExpressionBacterialMutationLetB(43-877; delta229-239, D228G, L240G)-6xHisPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
UKNP1.7.1
Plasmid#97371PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
MAGI1 gRNA (BRDN0001146833)
Plasmid#78067Purpose3rd generation lentiviral gRNA plasmid targeting human MAGI1DepositorInsertMAGI1 (MAGI1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAGI1 gRNA (BRDN0001487062)
Plasmid#78068Purpose3rd generation lentiviral gRNA plasmid targeting human MAGI1DepositorInsertMAGI1 (MAGI1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only