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pFBOH-MHL.RING1B

Plasmid

#224712

Purpose

Expresses N-terminal hexahistidine-tagged human RING1B (RNF2) in insect cells.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

RING1b (RNF2 Human)

Use

Tags

6x histidine tag

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Oct. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL.BMI1

Plasmid

#224711

Purpose

Expresses N-terminal hexahistidine-tagged human BMI1 (PCGF4) in insect cells.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

BMI1 (BMI1 Human)

Use

Tags

6x histidine tag

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-H230F

Plasmid

#222195

Purpose

Bacterial expression of mouse PTER-H230F protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

H230F

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-H26F

Plasmid

#222192

Purpose

Bacterial expression of mouse PTER-H26F protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

H26F

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST40-mPTER

Plasmid

#224864

Purpose

Mammalian expression mouse PTER protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

mouse PTER (Pter Mouse)

Use

Tags

C-term v5 and His tag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-E169A

Plasmid

#222190

Purpose

Bacterial expression of mouse PTER-E169A protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

E169A

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-D298A

Plasmid

#222191

Purpose

Bacterial expression of mouse PTER-D298A protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

D298A

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-H28F

Plasmid

#222193

Purpose

Bacterial expression of mouse PTER-H28F protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

H28F

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-H201F

Plasmid

#222194

Purpose

Bacterial expression of mouse PTER-H201F protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

H201A

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-R233A

Plasmid

#222197

Purpose

Bacterial expression of mouse PTER-R233A protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

R233A

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-H300A

Plasmid

#222198

Purpose

Bacterial expression of mouse PTER-H300A protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

H300A

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-L255A

Plasmid

#222199

Purpose

Bacterial expression of mouse PTER-L255A protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

L255A

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-L260G

Plasmid

#222200

Purpose

Bacterial expression of mouse PTER-L260G protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

L260G

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-T258G

Plasmid

#222201

Purpose

Bacterial expression of mouse PTER-T258G protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

T258G

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-R304K

Plasmid

#222202

Purpose

Bacterial expression of mouse PTER-R304K protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

R304K

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-Y65F

Plasmid

#222203

Purpose

Bacterial expression of mouse PTER-Y65F protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

Y65F

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-Y263F

Plasmid

#222204

Purpose

Bacterial expression of mouse PTER-Y263F protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

Y263F

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP PODXL delta PBM

Plasmid

#202415

Purpose

Expression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; doesn't bind NHERF1/2)

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

Lacking DTHL

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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B*46:01:01

Plasmid

#203250

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*46:01:01 (HLA-B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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B*53:01:01

Plasmid

#203249

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*53:01:01 (HLA-B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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