-
PurposeLevel 0 zCas9i vector
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 153221 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepAGM4031
-
Backbone manufacturerMarillonnet, IPB
- Backbone size w/o insert (bp) 2069
-
Vector typeSynthetic Biology
Growth in Bacteria
-
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha ; DH10B
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSpCas9 with introns and 2 NLS
-
SpeciesSynthetic
-
Insert Size (bp)5793
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
-
Supplemental Documents
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.04.03.023036v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAGM47523 was a gift from Sylvestre Marillonnet (Addgene plasmid # 153221 ; http://n2t.net/addgene:153221 ; RRID:Addgene_153221) -
For your References section:
High-efficiency genome editing in plants mediated by a Cas9 gene containing multiple introns. Grutzner R, Martin P, Horn C, Mortensen S, Cram EJ, Lee-Parsons CWT, Stuttmann J, Marillonnet S. Plant Commun. 2020 Nov 23;2(2):100135. doi: 10.1016/j.xplc.2020.100135. eCollection 2021 Mar 8. 10.1016/j.xplc.2020.100135 PubMed 33898975