Addgene

We narrowed to 4,146 results for: PRK

Showing: 621 - 640 of 4146 results

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pRK5-Myc-TEAD1-Y406A

Plasmid

#33047

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

TEAD1 (TEAD1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Y406A

Promoter

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pRK5-mEGFP-PHOX2B-MUT

Plasmid

#194577

Purpose

Mammalian Expression of mEGFP-PHOX2B Mutant (S207Afs*102) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

PHOX2B (PHOX2B Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

S207Afs*102

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-Puro- MLL3-PRKAG2

Plasmid

#69473

Purpose

Expresses MLL3-PRKAG2 fusion gene

Depositor

Axel Hillmer

Article

Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

Insert

MLL3-PRKAG2 (PRKAG2 Human)

Use

Retroviral

Tags

HA TAG:

Expression

Mutation

Promoter

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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HA GST p85 S6K1 pRK5

Plasmid

#8466

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

p85 s6k1 (Rps6kb1 Rat)

Use

Tags

ha/gst

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
pRK5-PLBD2-6xHis S392A

Plasmid

#213607

Purpose

Expresses PLBD2-6xHis S392A in mammalian cells

Depositor

Monther Abu-Remaileh

Article

Nyame et al Mol Cell. 2024 Apr 4;84(7):1354-1364.e9.

Insert

phospholipase B domain containing 2 (PLBD2 Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

Changed serine 392 to alanine

Promoter

CMV

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pRK-Myc-Smurf1 delHECT

Plasmid

#13677

Depositor

Ying Zhang

Article

Yamashita et al Cell. 2005 Apr 8. 121(1):101-13.

Insert

Smurf1 deltaHect (SMURF1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Smurf1 without HECT domain (deleted amino acids 3…

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only
pRK5-mEGFP-RAX-MUT

Plasmid

#194573

Purpose

Mammalian Expression of mEGFP-RAX Mutant (S222Rfs*63) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

RAX (RAX Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

S222Rfs*63

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-MYOD1-MUT

Plasmid

#194571

Purpose

Mammalian Expression of mEGFP-MYOD1 Mutant (R188Pfs*90) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

MYOD1 (MYOD1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

R188Pfs*90

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 CASTOR2-Flag Cterm

Plasmid

#84490

Purpose

overexpression of CASTOR2 with Flag tag

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR2 (GATSL2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro1 S432N

Plasmid

#47893

Purpose

Expresses myc tagged Miro1 S432N dominant negative mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro1 S432N (RHOT1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

S432N, dominant negative mutant

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-myc-PICK1-rescue

Plasmid

#72573

Purpose

Expresses myc-PICK1 shRNA resistant cDNA

Depositor

Richard Huganir, Victor Anggono

Article

Anggono et al J Neurosci. 2011 Feb 9;31(6):2188-96. do

Insert

PICK1 (Pick1 Rat)

Use

Tags

Myc

Expression

Mammalian

Mutation

5 silent mutations around shRNA recognition seque…

Promoter

CMV

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K27R-K29R

Plasmid

#121156

Purpose

Mammalian expression of HA tagged ubiquitin K27R and K29R

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Use

Tags

Expression

Mammalian

Mutation

K27 and K29 mutated to Rs

Promoter

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-GST-Presc-mAkt1 (wt)

Plasmid

#48805

Purpose

expresses wt Akt1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

Akt1 (Akt1 Mouse)

Use

Tags

HA-GST-Presc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-mEGFP-FOXF1-MUT

Plasmid

#194569

Purpose

Mammalian Expression of mEGFP-FOXF1 Mutant (A231Rfs*61) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

FOXF1 (FOXF1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

A231Rfs*61

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-Raptor K840R

Plasmid

#204418

Purpose

Acetylation mutant for human raptor

Depositor

David Rubinsztein

Article

Son et al Cell Metab. 2019 Jan 8;29(1):192-201.e7.

Insert

Regulatory Associated Protein Of MTOR, Complex 1

Use

Tags

HA and YFP

Expression

Mammalian

Mutation

Lysine 840 to Arginine (K840R)

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-Raptor K1097R

Plasmid

#204417

Purpose

Acetylation mutant for human raptor

Depositor

David Rubinsztein

Article

Son et al Cell Metab. 2019 Jan 8;29(1):192-201.e7.

Insert

Regulatory Associated Protein Of MTOR, Complex 1

Use

Tags

HA and YFP

Expression

Mammalian

Mutation

Lysine 1097 to Arginine (K1097R)

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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prk5-TMEM192-3xHA PM mutant

Plasmid

#149506

Purpose

Transient expression of human TMEM192. Dileucine motifs LL23-24 and LL28-29 have been mutated to AA.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

TMEM192 (TMEM192 Human)

Use

Transient expression

Tags

3XHA

Expression

Mammalian

Mutation

p.LL23-24AA, p.LL28-29AA

Promoter

CMV

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pRK DPC4 deltaC Flag

Plasmid

#12628

Depositor

Rik Derynck

Article

Zhang et al Nature. 1996 Sep 12. 383(6596):168-72.

Insert

DPC4 delta C (SMAD4 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

C-terminal truncated DPC4 (aa 1-511)

Promoter

Available since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pRK5-H1-shRNA-CMV-HA-PACSIN1

Plasmid

#72577

Purpose

Bicistronic construct expressing PACSIN1 shRNA and HA-PACSIN1-rescue construct

Depositor

Richard Huganir

Article

Anggono et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

Inserts

PACSIN1 (Pacsin1 Mouse)
H1-PACSIN1 shRNA cassette

Use

RNAi

Tags

HA

Expression

Mammalian

Mutation

5 silent mutations around shRNA recognition seque…

Promoter

CMV and H1

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag Grb10 5A short

Plasmid

#37484

Depositor

David Sabatini

Article

Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

Insert

Grb10 5A (GRB10 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

isoform C (missing N-term 58aa), 536aa, S150A T15…

Promoter

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only