Addgene

We narrowed to 14,749 results for: LEA

Showing: 6281 - 6300 of 14749 results

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mKate2-DrCavin1b in pT3TS-Dest

Plasmid

#194295

Purpose

In vitro transcription of mKate2 tagged zebrafish cavin1b from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAC

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1b (cavin1b Zebrafish)

Use

In vitro transcription of mrna

Tags

Expression

Mutation

Promoter

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pPBbleo-miRFP703-eDHFR(69K6)-Tiam1(DH-PH)

Plasmid

#186703

Purpose

Mammalian expression of Tiam1(DH-PH) fused to miRFP703-eDHFR(69K6)

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

miRFP703-eDHFR(69K6)-Tiam1(DH-PH)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pNHT7::UMSBP::sNluc2_ORF2

Plasmid

#186766

Purpose

An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at a start codon further downstream of the endogenous gene's start codon..

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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2xNLS-NLP-cMyc AspCas12a

Plasmid

#182120

Purpose

pET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

2xNLS-NLP-cMyc AspCas12a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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H2B-mNeonGreen_FRB(DmrC)_IRESpuro2

Plasmid

#182918

Purpose

Expesses H2B-mNeonGreen_FRB(DmrC). Can be used to target FKBP(DmrA) tagged proteins to H2B

Depositor

Daniel Gerlich

Article

Cuylen-Haering et al Nature. 2020 Nov;587(7833):285-290. doi:

Insert

pH2B (H2BC16P Human)

Use

Tags

mNeonGreen-FRB(DmrC)

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TetO(3G)-GCaMP6f-WPRE

Plasmid

#186205

Purpose

Express GCaMP6f under TetO(3G)

Depositor

Naoshige Uchida

Article

Amo et al Nat Neurosci. 2022 Aug;25(8):1082-1092.

Insert

GCaMP6f

Use

AAV

Tags

6xHis (N terminal on insert), T7 epitope, and Xpr…

Expression

Mutation

Promoter

TetO(3G)

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLX_HA-C7orf26v2

Plasmid

#178536

Purpose

Subcloned C7orf26v2 (ENST00000359073, Broad GPP TRCN0000477172) into pLX_HA backbone

Depositor

William Hahn

Article

Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

Insert

C7orf26v2 (INTS15 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAT15541_dCBE-SuperFi-Cas9

Plasmid

#184371

Purpose

Mammalian expression of nuclease inactive SuperFi-Cas9 CBE base editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

dCBE-SuperFi-Cas9

Use

CRISPR

Tags

3X FLAG, SV40 NLS, and UGI

Expression

Mammalian

Mutation

D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

Promoter

EF-1α core promoter

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAT15543_dABE-SuperFi-Cas9

Plasmid

#184373

Purpose

Mammalian expression of nuclease inactive SuperFi-Cas9 ABE7 base editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

dABE-SuperFi-Cas9

Use

CRISPR

Tags

FLAG, SV40 NLS, and nucleoplasmin NLS

Expression

Mammalian

Mutation

D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

Promoter

EF-1α core promoter

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAT15545_dABE8e-SuperFi-Cas9

Plasmid

#184375

Purpose

Mammalian expression of nuclease inactive SuperFi-Cas9 ABE8e base editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

dABE8e-SuperFi-Cas9

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Mutation

D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

Promoter

EF-1α core promoter

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLX_HA-C7orf26v1

Plasmid

#178535

Purpose

Subcloned C7orf26v1 (ENST00000344417, Origene, RC219786L4) into pLX_HA backbone

Depositor

William Hahn

Article

Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

Insert

C7orf26v1 (INTS15 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot7-M141R_R

Plasmid

#147379

Purpose

Mammalian Expression of HsNot7-M141R

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot7-M141R (CNOT7 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NvG-HsNot7_9-260_R

Plasmid

#147369

Purpose

Bacterial Expression of HsNot7_9-260

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot7_9-260 (CNOT7 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Tmx4-V5 C209A

Plasmid

#175140

Purpose

Lentiviral expression of V5-tagged mouse Tmx4

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmx4 (Tmx4 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

C209A

Promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Tmx4-V5 C211A

Plasmid

#175139

Purpose

Lentiviral expression of V5-tagged mouse Tmx4

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmx4 (Tmx4 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

C211A

Promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Hsd11b1-V5

Plasmid

#175147

Purpose

Lentiviral expression of mouse Hsd11b1-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Hsd11b1 (Hsd11b1 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Tmx4-V5 C209A, C211A

Plasmid

#175141

Purpose

Lentiviral expression of V5-tagged mouse Tmx4

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmx4 (Tmx4 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

C209A, C211A

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pEZT-GST-SLFN2-T1

Plasmid

#174320

Purpose

Expression of recombinant GST-SLFN2-R67A, K68A, N71A

Depositor

Bruce Beutler

Article

Yue et al Science. 2021 May 14;372(6543). pii: 372

Insert

GST-SLFN2-R67A, K68A, N71A (Slfn2 Mouse)

Use

Bacmam, baculovirus

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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EC2_2_dCas9_VP64_sgRNA

Plasmid

#163707

Purpose

Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
VP64+SV40 NLS

Use

Tags

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pEZT-GST-SLFN2-T2

Plasmid

#174321

Purpose

Expression of recombinant GST-SLFN2-K242A, K249A, Q301A

Depositor

Bruce Beutler

Article

Yue et al Science. 2021 May 14;372(6543). pii: 372

Insert

GST-SLFN2-K242A, K249A, Q301A (Slfn2 Mouse)

Use

Bacmam, baculovirus

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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