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Plasmid#197507PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HF1(D10A) and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-ABE8e-nSpRY-HF1-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA and nSpRY-HF1(D10A/A61R/N…PromoterCMV and T7Available sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
LC-E75
Bacterial Strain#115925PurposeExpression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosomeDepositorBacterial ResistanceNoneAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
phR-Luc
Plasmid#139644PurposeMammalian gene expressionDepositorInsertRenilla luciferase
UseLuciferaseTagsExpressionMutationPromoterSV40Available sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.hSyn.Cre.WPRE.hGH (AAV8)
Viral Prep#105553-AAV8PurposeReady-to-use AAV8 particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA. hSyn-driven Cre expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1‐hsRNASEH2BCA
Plasmid#108692PurposeFor expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzymeDepositorUseTagsGSTExpressionBacterialMutationShine Dalgarno sequence upstream of start codon a…PromotertacAvailable sinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1‐hsRNASEH2BCA
Plasmid#108692PurposeFor expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzymeDepositorUseTagsGSTExpressionBacterialMutationShine Dalgarno sequence upstream of start codon a…PromotertacAvailable sinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVoPo-01
Plasmid#187836PurposeBackbone for transcriptional reporter system in Fusobacterium nucleatumDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.S
Plasmid#103006Purposenon-standard AAV2 rep-AAV-PHP.S cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.S VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.S
Plasmid#103006Purposenon-standard AAV2 rep-AAV-PHP.S cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.S VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFX-mito-EGFP
Plasmid#159096PurposeExpress EGFP with mitochondria-targeting sequence in mammalian cellsDepositorInsertmCherry with mitochondria-targeting sequence
UseTagsthe mitochondria-targeting sequence of the cytoch…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin
Plasmid#133027PurposeExpresses EGFP-tagged late endosome markerDepositorInsertRAB7A (RAB7A Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterHuman elongation factor 1 alphaAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
Bassik lab Human CRISPR-Cas9 Deletion Library - Drug targets, kinases, phosphatases
Pooled Library#101927PurposeBassik lab Human CRISPR-Cas9 Deletion Library - Drug targets, kinases, phosphatases. Coexpresses mCherry.DepositorExpressionMammalianUseCRISPRAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI-SEP-GluR1
Plasmid#24000DepositorInsertGluR1 (Gria1 Rat)
UseTagsGluR1 signal peptide (aa1-22) and SEP (supereclip…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCI-SEP-GluR1
Plasmid#24000DepositorInsertGluR1 (Gria1 Rat)
UseTagsGluR1 signal peptide (aa1-22) and SEP (supereclip…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLX304 Flag-mCherry-eEF1A1_IRES-GFP
Plasmid#198383PurposeeEF1A1 fluorescent reporterDepositorInserteEF1A1 (EEF1A1 Human)
UseLentiviralTagsFlag-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFD152
Plasmid#125546Purposederivative of pFD116 with an optimized RBS for E. coliDepositorInsertoptimized RBS
UseCRISPRTagsExpressionMutationPromoteranhydrotetracycline Ptet promoterAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS:CoChR-tdTomato
Plasmid#124233PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomatoDepositorInsertCoChR-tdTomato
UseZebrafish expressionTagstdTomatoExpressionMutationPromoter14xUAS-E1bAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xAP1pGL3 (3xAP-1 in pGL3-basic)
Plasmid#40342DepositorInsert3xAP-1
UseLuciferaseTagsLuciferaseExpressionMammalianMutationContains three canonical AP-1 binding sites (TGAC…PromoterAvailable sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
3xAP1pGL3 (3xAP-1 in pGL3-basic)
Plasmid#40342DepositorInsert3xAP-1
UseLuciferaseTagsLuciferaseExpressionMammalianMutationContains three canonical AP-1 binding sites (TGAC…PromoterAvailable sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-cMSP1E3D1
Plasmid#199772PurposeExpresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)DepositorInsertcMSP1E3D1
UseTagsHis6-TagExpressionBacterialMutationExtended cMSP1D1; external His-Tags flanking the …PromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only