pFX-mito-EGFP
(Plasmid
#159096)
-
PurposeExpress EGFP with mitochondria-targeting sequence in mammalian cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 159096 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepFX
- Backbone size w/o insert (bp) 3979
- Total vector size (bp) 4843
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namemCherry with mitochondria-targeting sequence
-
Insert Size (bp)864
-
GenBank ID
- Promoter CMV
-
Tag
/ Fusion Protein
- the mitochondria-targeting sequence of the cytochrome c oxidase subunit VIII (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhiI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer none
- 3′ sequencing primer none (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pFX-mito-EGFP was a gift from Yusuke Ohba (Addgene plasmid # 159096 ; http://n2t.net/addgene:159096 ; RRID:Addgene_159096) -
For your References section:
Folding Latency of Fluorescent Proteins Affects the Mitochondrial Localization of Fusion Proteins. Kashiwagi S, Fujioka Y, Satoh AO, Yoshida A, Fujioka M, Nepal P, Tsuzuki A, Aoki O, Paudel S, Sasajima H, Ohba Y. Cell Struct Funct. 2019 Dec 26;44(2):183-194. doi: 10.1247/csf.19028. Epub 2019 Nov 15. 10.1247/csf.19028 PubMed 31735740