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pGR618
Plasmid#213786PurposeIntegrates at the CAN1 locus the BB-Tv TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassetteDepositorInsertBB-Tv TP-DNAP1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-FES
Plasmid#203501PurposeExpresses human FES kinase from a GAL promoter with a URA3 markerDepositorInsertFES (FES Human)
UseTagsExpressionYeastMutationPromoterGAL1Available sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GALL-SARS-CoV-2_3CL
Plasmid#203496PurposeExpresses SARS-CoV-2 3CL protease from a GALL promoter with a URA3 markerDepositorInsertSARS-CoV-2 3CL protease (ORF1ab )
UseTagsExpressionYeastMutationPromoterGALLAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-2x-uA-BWCoV-SW1_3CL
Plasmid#201936PurposeExpresses Beluga Whale-CoV SW1 3CL protease from a GAL-2x promoter with a URA3 markerDepositorInsertBeluga Whale-CoV SW1 3CL protease
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-Bat-CoV-HKU4_3CL
Plasmid#201943PurposeExpresses Bat-CoV HKU4 3CL protease from a GAL10 promoter with a URA3 markerDepositorInsertBat-CoV HKU4 3CL protease (ORF1ab )
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
HCP4
Plasmid#166106PurposeThis plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the center of Cyr1 and the other targets a non-coding region on chromosome X (location X1)DepositorInsertCyr1Del-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP6
Plasmid#166108PurposeThis plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Cyr1 and the other targets a non-coding region on chromosome X (location X1)DepositorInsertCyr1CT-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGAD424-hL1
Plasmid#89423PurposeTwo-hybrid systemDepositorInsertL1CAM (L1CAM Human)
UseTagsGal4 ADExpressionYeastMutationPromoterADH1Available sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS2-mCALEBsplice.cyt (aa 465-566)
Plasmid#89410PurposeTwo-hybrid systemDepositorInsertCSPG5 (Cspg5 Mouse)
UseTagsGAL4 DBDExpressionYeastMutationaa 465-566PromoterADH1Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS2-mCALEBlong.cyt (aa 522-594)
Plasmid#89409PurposeTwo-hybrid systemDepositorInsertCSPG5 (Cspg5 Mouse)
UseTagsGAL4 DBDExpressionYeastMutationaa 522-594PromoterADH1Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS2-mCALEBcyt (aa 465-539)
Plasmid#89408PurposeTwo-hybrid systemDepositorInsertCSPG5 (Cspg5 Mouse)
UseTagsGAL4 DBDExpressionYeastMutationaa 465-539PromoterADH1Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS2-chCALEBcyt (aa 522-551)
Plasmid#89407PurposeTwo-hybrid systemDepositorInsertCSPG5 (CSPG5 Chicken)
UseTagsGAL4 DBDExpressionYeastMutationaa 522-551PromoterADH1Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-mCALEBsplice (aa 447-566)
Plasmid#89405PurposeTwo-hybrid systemDepositorInsertCSPG5 (Cspg5 Mouse)
UseTagsGAL4 DBDExpressionYeastMutationPromoterADH1Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T2-Ste7-EE
Plasmid#52681Purposeexpression of constitutively active Ste7 kinase in bacteriaDepositorInsertSte7 (STE7 Budding Yeast)
UseTagsGST and MycExpressionBacterialMutationS359E/T363E constitutively activePromotertacAvailable sinceApril 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYX233-b2delta-DHFR
Plasmid#163760PurposeGal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)delta(19)-DHFRDepositorInsertb2delta-DHFR (Dhfr Budding Yeast, Mouse)
UseTagsExpressionYeastMutationAmino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…PromoterGAL1Available sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-C1
Plasmid#125549PurposeGateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment1DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment1 attached to c-terminus of Gatew…ExpressionMammalian and YeastMutationPromotersynthetic promoter containing yeast, mammalian, a…Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-C2
Plasmid#125559PurposeGateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment2DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment2 attached to c-terminus of Gatew…ExpressionMammalian and YeastMutationPromotersynthetic promoter containing yeast, mammalian, a…Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-N2H-N2
Plasmid#125548PurposeGateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment2DepositorTypeEmpty backboneUseIn vitro expressionTagsNanoLuc fragment2 attached to n-terminus of Gatew…ExpressionMammalian and YeastMutationPromotersynthetic promoter containing yeast, mammalian, a…Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only
