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Plasmid#222212PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the W15C mutation and a C-terminal His tagDepositorInsertfghA(W15C)
UseTags6x HisExpressionBacterialMutationChanged Tryptophan 15 to CysteinePromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-WT1 +KTS N terminal HA
Plasmid#217639PurposeEntry vector with WT1 +KTS mutant with N terminal HA for gateway cloningDepositorInsertWT1 +KTS isoform (WT1 Human)
UseGateway cloningTagsHAExpressionMutationPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-WT1 +KTS C terminal HA
Plasmid#217640PurposeEntry vector with WT1 +KTS mutant with C terminal HA for gateway cloningDepositorInsertWT1 +KTS isoform (WT1 Human)
UseGateway cloningTagsHAExpressionMutationPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-WT1 -KTS N terminal HA
Plasmid#217641PurposeEntry vector with WT1 -KTS mutant with N terminal HA for gateway cloningDepositorInsertWT1 -KTS isoform (WT1 Human)
UseGateway cloningTagsHAExpressionMutationp.408_410delKTSPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFP-WT1 -KTS C terminal HA
Plasmid#217642PurposeEntry vector with WT1 -KTS mutant with C terminal HA for gateway cloningDepositorInsertWT1 -KTS isoform (WT1 Human)
UseGateway cloningTagsHAExpressionMutationp.408_410delKTSPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 genomic A. thaliana PLDGAMMA3
Plasmid#209188PurposepENTR4 plasmid with genomic sequence of Arabidopsis thaliana PHOSPHOLIPASE D GAMMA 3 (PLDGAMMA3, AT4G11840) without stop codon for C-terminal epitope tagsDepositorInsertAT4G11840 (PLDGAMMA3 Mustard Weed)
UseGateway-compatible entry vectorTagsExpressionMutationPromoternoneAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW pHluorin-SYT9 IRES mRuby3
Plasmid#195698PurposeLentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoterDepositorInsertSyt9 (Syt5 Rat)
UseLentiviralTagspHluorinExpressionMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW Syt9 IRES mRuby3
Plasmid#195700PurposeLentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoterDepositorInsertSyt9 (Syt5 Mouse)
UseLentiviralTagsExpressionMutationencodes only the first 68 amino acids of Tac1PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBMN HA-ATG13(W50D)
Plasmid#188639PurposeFor expression of human ATG13(W50D)DepositorInsertATG13 (ATG13 Human)
UseRetroviralTagsHAExpressionMammalianMutationChanged tryptophan 50 to aspartic acidPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-CBE1
Plasmid#195367PurposedLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN with 3xFLAGDepositorInserthA3A/Y130F-XTEN with 3xFLAG-dLbCas12a-D156R-UGI
UseCRISPR; Gateway compatible ha3a/y130f-xten plus 3…Tags3xflagExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
KTK_005
Plasmid#180555PurposeEntry vector containing Terminator sequence (Bba_B0015)DepositorInsertTerminator sequence (Bba_B0015)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 Ta-SRO1 JN177
Plasmid#192546PurposepENTR plasmid Triticum aestivum SRO1, full CDS, cultivar JN177, no stop codonDepositorInsertSIMILAR TO RCD ONE 1 (SRO1), cultivar Jinan 177 (JN177)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 Ta-sro1 SR3
Plasmid#192547PurposepENTR plasmid Triticum aestivum sro1, full CDS, cultivar SR3, no stop codonDepositorInsertSIMILAR TO RCD ONE 1 (SRO1), cultivar Shanrong No. 3 (SR3)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUBI-RZ-Cas12j2
Plasmid#189781PurposeCas12j2 (CasΦ) Gateway gRNA entry plasmid using Zea mays Ubi promoter and HH, HDV ribozyme processingDepositorInsertgRNA cloning site
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-Ark-L2
Plasmid#186362PurposeEntry clone with ORF encoding Ciona robusta Aurora kinase A flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.DepositorInsertCiona robusta Aurora kinase A
UseExpression of a fluorescent centrosome markerTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-HistoneH2B-L2
Plasmid#186357PurposeEntry clone with ORF encoding histone H2B flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.DepositorInsertHistone H2B (H2BC11 Human)
UseExpression of a tagged nuclear markerTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_047
Plasmid#180551PurposeEntry vector containing sfGFP in E1.3a formatDepositorInsertsfGFP in E1.3a format
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
eDrIR1
Plasmid#183998PurposeOptically controlled eDrIR1 expression in mammalian cellsDepositorInserteDrIR1 (INSR Human)
UseTagsIg kappa secretory signal, mCherry, Deinococcus r…ExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_110
Plasmid#180554PurposeEntry vector containing 7xhis tag in E1.3b formatDepositorInsert7xhis tag in E1.3b format
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_007
Plasmid#180563PurposeEntry vector containing Terminator sequence (Bba_B0015)DepositorInsertTerminator sequence (Bba_B0015)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only