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Plasmid#132301PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC7A3 (SLC7A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS2+-HypocratesCS
Plasmid#161960PurposeMammalian expression of non-targeted inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivativesDepositorInsertHypocrates
UseTagsExpressionMammalianMutationchanged Cysteine 355 to SerinePromoterCMV, SP6Available sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-TlcnC
Plasmid#114376Purposeexpresses Flpo recombinase-dependent GtACR2-Kv2.1C-TlcnC, which targets GtACR2 to the somatodendritic compartmentDepositorInsertGtACR2-EYFP-Kv2.1C-TlcnC (NEWENTRY )
UseAAVTagsEYFP, Kv2.1C, and TlcnCExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-MCS-QF-MiniWhite
Plasmid#165906PurposeBlasticidin resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloningDepositorInsertSelectable Empty Enhancer Driver
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEntr1a-mcherry-FKBP-TIAM1(DHPH)
Plasmid#176135PurposeHeterodimerization with FRB (rapamycin), Rac1 activationDepositorInsertTIAM1 (TIAM1 Human)
UseEntry vectorTagsExpressionMutationSee Depositor Comments BelowPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR CMVie-Lifeact-iRFP670 L1-R5
Plasmid#84390PurposeMultisite Gateway entry vector for expression of iRFP670-tagged Lifeact in cells including neuronsDepositorInsertLifeact
UseGatewayTagsiRFP670ExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR P4-P1R-V5-6xHis
Plasmid#48346PurposeContributes the coding sequence of a V5-6xHis epitope tags cassette as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertV5 and 6xHis epitope tags cassette
UseGateway entry cloneTagsExpressionMutationContains a Kozak sequence and initiation codon up…PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-DNM2-W525L
Plasmid#155263PurposeMammalian expression of rat DNM2 fused to HADepositorInsertdynamin 2 (Dnm2 Rat)
UseTagsHAExpressionMammalianMutationTryptophan 525 to LeucinePromoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-DNM2-R465W
Plasmid#155262PurposeMammalian expression of rat DNM2 fused to HADepositorInsertdynamin 2 (Dnm2 Rat)
UseTagsHAExpressionMammalianMutationArginine 465 to TryptophanPromoterAvailable sinceMay 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pG418R-MCS-QF-MiniWhite
Plasmid#165902PurposeG418 resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloningDepositorInsertSelectable Empty Enhancer Driver
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
mILK-L207W-EFGP
Plasmid#176900Purposemouse ILK with L207W point mutation cloned into pEGFP-N1DepositorInsertIntegrin-linked kinase (Ilk Mouse)
UseTagsEGFPExpressionMammalianMutationLeucine 207 to TryptophanePromoterCNVAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(W66)
Plasmid#63217PurposeExpression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(W66)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
HBM-mVEGF-C-HisTag-pENTR
Plasmid#64066PurposePlasmid encodes mature form of murine VEGF-C preceded by honey bee melittin secretion signal; entry vector to Gateway cloning system.DepositorInsertvascular endothelial growth factor C (Vegfc Mouse)
UseTagsHis-tagExpressionInsectMutationPromoterAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-STU-Fn
Plasmid#138104PurposeGolden Gate entry vector for 4th crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131-STU-Fn
Plasmid#138095PurposeGolden Gate entry vector for 1st crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132-STU-Fn
Plasmid#138098PurposeGolden Gate entry vector for 2nd crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133-STU-Fn
Plasmid#138101PurposeGolden Gate entry vector for 3rd crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_DW164AA USP7
Plasmid#131252PurposeMammalian expression of N-terminally Myc-tagged DW164AA USP7DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
UseTagsMycExpressionMammalianMutationContains a point mutation that converts USP7 aspa…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR-mDIXDC1 WT
Plasmid#61216PurposeEntry clone encoding long isoform of mouse DIXDC1DepositorInsertDIXDC1 (Dixdc1 Mouse)
UseDonr clone (gateway)TagsExpressionMutationPromoterAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW/TOPO-TBX20a
Plasmid#98620PurposeGateway entry TOPO TA cloning vector for TBX20aDepositorInsertTbx20a (Tbx20 Mouse)
UseTopo ta cloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only