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Plasmid#181706PurposeGalactose inducible expression of RNQ1DepositorInsertRNQ1 (RNQ1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-YES1-6stop
Plasmid#203476PurposeExpresses human YES1 kinase from a GAL10 promoter with a URA3 markerDepositorInsertYES1 (YES1 Human)
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-Bat-SL-CoVZC45_PLP
Plasmid#201939PurposeExpresses Bat-SL-CoVZC45 papain-like protease from a GAL promoter with a URA3 markerDepositorInsertBat-SL-CoVZC45 PLP
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-HCV_NS3-GS-NS4A
Plasmid#203504PurposeExpresses HCV NS3-NS4A protease (with GS linker) from a GAL promoter with a URA3 markerDepositorInsertHCV NS3-GS-NS4A
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-HRV-B14_3C
Plasmid#203466PurposeExpresses HRV-B14 3C protease from a GAL10 promoter with a URA3 markerDepositorInsertHRV-B14 3C protease (HRVBgp1 )
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-DUGV_OTU
Plasmid#203463PurposeExpresses DUGV OTU domain of L protein from a GAL10 promoter with a URA3 markerDepositorInsertDUGV OTU domain of L protein (DUGVsLgp1 )
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-HCoV-NL63_PLP2
Plasmid#203502PurposeExpresses HCoV-NL63 PLP2 from a GAL promoter with a URA3 markerDepositorInsertHCoV-NL63 PLP2 (ORF1ab )
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-cPr-mNeonGreen (ErmR)
Plasmid#177208PurposePlasmid with p15A origin for constitutive mNeonGreen expression in E. coliDepositorInsertmNeonGreen
UseTagsExpressionBacterialMutationYeast codon optimizedPromoterSynthetic constitutive promoterAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
HCP1
Plasmid#166103PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination.DepositorInsertMsn2-sgRNA#27 (MSN2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK138
Plasmid#73314PurposePas2p peroxisomal membrane targeting sequenceDepositorInsertPas2p (peroxisomal membrane targeting region)
UseTagsHis6 and c-mycExpressionYeastMutationencodes residues 1-42PromoterAOX1Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
brr2-R1107M pRS313
Plasmid#111417Purposebrr2-R1107M (endogenous promoter)DepositorInsertBRR2
UseTagsExpressionYeastMutationR1107M aa mutation (note, not an RP allele)PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAG303-Gal-PR50
Plasmid#84905Purposeinduce expression of dipeptide repeat PR in yeastDepositorInsertPR50 (C9orf72 Synthetic)
UseTagsFLAG and MycExpressionYeastMutationPromoterGAL1Available sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYX233-b2-DHFR
Plasmid#163759PurposeGal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)-DHFRDepositorInsertb2-DHFR (Dhfr Budding Yeast, Mouse)
UseTagsExpressionYeastMutationAmino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…PromoterGAL1Available sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG303-Gal-GR100
Plasmid#84908Purposeinduce expression of dipeptide repeat GR in yeastDepositorInsertGR100 (C9orf72 Synthetic)
UseTagsExpressionYeastMutationPromoterGAL1Available sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNK5712
Plasmid#219752PurposepGAP-like vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of GAP promoter, for yeast expressionDepositorInsertpGAP - nnH3H_v2 - tAOX
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK5785
Plasmid#219753PurposepGAP-like vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of GAP promoter, for yeast expressionDepositorInsertpGAP - nnLuz_v4 - tAOX
UseLuciferaseTagsExpressionYeastMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-Kss1
Plasmid#52682PurposeExpression of Kss1 in bacteriaDepositorInsertKSS1 (KSS1 Budding Yeast)
UseTagsHis6 and T7 tagExpressionBacterialMutationPromoterT7Available sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYeDP60-hATP13A2-WT
Plasmid#171377PurposeA modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888DepositorInserthuman ATP13A2 variant 2 wild-type
UseTagsBAD tagExpressionYeastMutationPromoterURA3Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
DHC1-AID-Clover donor (Hygro)
Plasmid#158622PurposeDonor plasmid for tagging DHC1 with AID-CloverDepositorInsertDHC1 (DYNC1H1 Human)
UseCRISPR; Tagging donorTagsExpressionMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG416-Gal-GA50
Plasmid#84903Purposeinduce expression of dipeptide repeat GA in yeastDepositorInsertGA50 (C9orf72 Synthetic)
UseTagsFLAG and MycExpressionYeastMutationPromoterGAL1Available sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only