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Addgene

pET28-Kss1
(Plasmid #52682)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 52682 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    KSS1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1100
  • GenBank ID
    852931
  • Entrez Gene
    KSS1 (a.k.a. YGR040W)
  • Promoter T7
  • Tags / Fusion Proteins
    • His6 (N terminal on backbone)
    • T7 tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

When co-expressed with Ste7EE (addgene plasmid #52681), produces active phosphorylated Kss1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-Kss1 was a gift from Benjamin Turk (Addgene plasmid # 52682 ; http://n2t.net/addgene:52682 ; RRID:Addgene_52682)
  • For your References section:

    Deciphering protein kinase specificity through large-scale analysis of yeast phosphorylation site motifs. Mok J, Kim PM, Lam HY, Piccirillo S, Zhou X, Jeschke GR, Sheridan DL, Parker SA, Desai V, Jwa M, Cameroni E, Niu H, Good M, Remenyi A, Ma JL, Sheu YJ, Sassi HE, Sopko R, Chan CS, De Virgilio C, Hollingsworth NM, Lim WA, Stern DF, Stillman B, Andrews BJ, Gerstein MB, Snyder M, Turk BE. Sci Signal. 2010 Feb 16;3(109):ra12. doi: 10.1126/scisignal.2000482. 10.1126/scisignal.2000482 PubMed 20159853