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Showing: 6081 - 6100 of 16077 results

  1. pANY4-pL18-ccdB

    Plasmid
    #164898
    Purpose
    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    164898-plasmid-map-sequence-id-327989

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  2. pBABE.puro/TO/Flag/mKO2

    Plasmid
    #110353
    Purpose
    Doxycycline inducible mammalian retroviral expression of mKO2 with FLAG tag
    Depositor
    Sandra Martha Gomes Dias
    Insert
    monomeric Kusabira Orange 2
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    gag
    Availability
    Academic Institutions and Nonprofits only
    110353-plasmid-map-sequence-id-254685

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  3. pET28-H12TEV

    Plasmid
    #208635
    Purpose
    for E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    6xhis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    208635-plasmid-map-sequence-id-433640

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  4. pSLIK 3xFLAG-NUP37 neo

    Plasmid
    #220361
    Purpose
    Lentiviral expression vector for an inducible 3xFLAG-tagged NUP37
    Depositor
    Kevin Janes
    Insert
    NUP37 (NUP37 Human)
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    220361-plasmid-map-sequence-id-436885

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  5. LEMD3_BirA_Cterm

    Plasmid
    #221517
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Lemd3
    Depositor
    Alessandro Ori
    Insert
    LEMD3 (LEMD3 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    221517-plasmid-map-sequence-id-439380

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  6. LEMD3_BirA_Nterm

    Plasmid
    #221518
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Lemd3
    Depositor
    Alessandro Ori
    Insert
    LEMD3 (LEMD3 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    221518-plasmid-map-sequence-id-439442

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  7. pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro

    Plasmid
    #72611
    Purpose
    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with triple alanine mutations on aminoacids#33,#34 and #37
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA binding protein 6 (GATA6 Human)
    Use
    Retroviral
    Tags
    3XAU1 and 3XFLAG
    Expression
    Mammalian
    Mutation
    changed serine33, threonine34 and serine37 to ala…
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  10. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMA-KO)

    Plasmid
    #153440
    Purpose
    E. coli expression of Rc. XDocB binding mode A knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc. XDocB binding mode A knock-out mutant
    Use
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Mutation
    Arginine 191 and leucine 195 on XDocB were mutate…
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    153440-plasmid-map-sequence-id-306440

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  18. ST-2xTstem-GFP

    Plasmid
    #162503
    Purpose
    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One Tac stem region is inserted within ST and the other Tac stem region is inserted between ST and GFP.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    GFP tagged ST6Gal1 mutant (ST6GAL1, IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    One Tac stem region is inserted within ST6Gal1 an…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    162503-plasmid-map-sequence-id-323511

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  19. ST-2xTstem(5A)-GFP

    Plasmid
    #162504
    Purpose
    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One wildtype Tac stem region is inserted within ST and the other mutated Tac stem region is inserted between ST and GFP.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    GFP tagged ST6Gal1 mutant (ST6GAL1, IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    One Tac stem region is inserted within ST6Gal1 an…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    162504-plasmid-map-sequence-id-323646

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  20. CAGGS-safb∆dd1-NLS-3XFLAG-V5

    Plasmid
    #196092
    Purpose
    Expresses mouse SAFB lacking the first computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag
    Depositor
    Mauro Calabrese
    Article
    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10
    Insert
    SAFB (Safb Mouse)
    Use
    Tags
    C-terminal 3xFLAG and C-terminal V5
    Expression
    Bacterial and Mammalian
    Mutation
    Removed amino acids 96-285 (predicted disordered …
    Promoter
    CAGGS
    Availability
    Academic Institutions and Nonprofits only
    196092-plasmid-map-sequence-id-384633

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Showing: 6081 - 6100 of 16077 results