pET28-H12TEV
(Plasmid
#208635)
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Purpose(Empty Backbone) for E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208635 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a(+)
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Backbone manufacturerMerck-Millipore (Novagen)
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Modifications to backboneadd another 6x his
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Vector typeBacterial Expression
- Promoter T7
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Tags
/ Fusion Proteins
- 6xhis (N terminal on backbone)
- 6xhis (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionswe use BL21 (DE3) for expression
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Copy numberLow Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28-H12TEV was a gift from Quanfu Ma (Addgene plasmid # 208635 ; http://n2t.net/addgene:208635 ; RRID:Addgene_208635)