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Addgene

pET28-H12TEV
(Plasmid #208635)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208635 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET28a(+)
  • Backbone manufacturer
    Merck-Millipore (Novagen)
  • Modifications to backbone
    add another 6x his
  • Vector type
    Bacterial Expression
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xhis (N terminal on backbone)
    • 6xhis (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    we use BL21 (DE3) for expression
  • Copy number
    Low Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-H12TEV was a gift from Quanfu Ma (Addgene plasmid # 208635 ; http://n2t.net/addgene:208635 ; RRID:Addgene_208635)