pcDNA8-Flag-LARS-DELTA-UNE-L
(Plasmid
#139691)
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PurposeExpresses N-teminal Flag tagged aa 1- 1064 of LARS1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 139691 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDNA3-FLAG TAGGED
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Backbone manufacturerFlag inserted and cloning site modified in lab
- Backbone size w/o insert (bp) 5400
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Modifications to backboneCMV promoter - T7 promoter - HindI - NotI - Kozak sequence - Flag - BamHI - LRS-XhoI…
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLARS1
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Alt nameLeucyl-tRNA-synthetase
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Alt nameLRS
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SpeciesH. sapiens (human)
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Insert Size (bp)3192
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Entrez GeneLARS1 (a.k.a. HSPC192, ILFS1, LARS, LEURS, LEUS, LFIS, LRS, PIG44, RNTLS, cLRS, hr025Cl)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-REV (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA8-Flag-LARS-DELTA-UNE-L was a gift from Jie Chen (Addgene plasmid # 139691 ; http://n2t.net/addgene:139691 ; RRID:Addgene_139691) -
For your References section:
Leucyl-tRNA Synthetase Activates Vps34 in Amino Acid-Sensing mTORC1 Signaling. Yoon MS, Son K, Arauz E, Han JM, Kim S, Chen J. Cell Rep. 2016 Aug 9;16(6):1510-1517. doi: 10.1016/j.celrep.2016.07.008. Epub 2016 Jul 28. 10.1016/j.celrep.2016.07.008 PubMed 27477288