-
Plasmid#153261PurposeM6E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only
-
pDGE664
Plasmid#153262PurposeM7 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE665
Plasmid#153263PurposeM8E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE654
Plasmid#153252PurposeM1E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE657
Plasmid#153255PurposeM2E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE659
Plasmid#153257PurposeM4 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE661
Plasmid#153259PurposeM5 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shDyn2
Plasmid#132712PurposeEncodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Pdyn geneDepositorInsertshDyn(2) (Pdyn Rat)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
E1b-GFP-Tol2-Gateway dre SE-zic2a P
Plasmid#89385PurposeVector for reporter assay of one zebrafish super-enhancer regionDepositorInsertzic2a (zic2a Zebrafish)
UseTagsExpressionMutationPromoterAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
MTK1_024
Plasmid#123686PurposeEncodes ConL2 and human C3 insulator as a Type 1 part to be used in the MTK systemDepositorInsertConnector L2-C3 insulator
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3_027
Plasmid#123742PurposeEncodes bicistronic ABI gene expression system as a Type 3 part to be used in the MTK systemDepositorInsertVP16::PYLcs-T2A-GAL4DBD::ABIcs
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3_YPet-PCP (y-plasmid)
Plasmid#127637PurposeEncodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034.DepositorInsertYPet
UseSynthetic BiologyTagsRNA binding peptide: PP7 coat protein (PCP)ExpressionBacterialMutationPromoterpJ21306Available sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
Keasling-2337
Plasmid#100168PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertlacIq-Ptrc-ADS
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
MTK1_023
Plasmid#123685PurposeEncodes ConL1 and human B1 insulator as a Type 1 part to be used in the MTK systemDepositorInsertConnector L1- B1 insulator
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_zeo
Plasmid#184917PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.DepositorInsertgRNA scaffold
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
MTK2_008
Plasmid#123703PurposeEncodes human pUbc as a Type 2 part to be used in the MTK systemDepositorInsertphUbC
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC023 - CaCas13a from [Clostridium] aminophilum DSM 10710
Plasmid#91913PurposeExpresses CaCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertCaCas13a and guide expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYL322d1/Vn-C
Plasmid#183154PurposeBimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vn.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXPR-SF61
Plasmid#163916PurposetRNA synthetase/tRNA pair for the in vivo incorporation of para-Pentafluorosulfanyl-Phenylalanine, into proteins in E. coli in response to the amber (TAG) codonDepositorInsertSF5Phe tRNA synthetase
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
PXNGW
Plasmid#48258PurposeSPLIT-GFP assay in plantsDepositorTypeEmpty backboneUseBinary gateway vectorTags5xHIS and YFP aa1-172ExpressionMutationPromoter35SAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only