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Addgene
Showing: 581 - 600 of 1659 results

  4. pcDNA3.1 NKX6-2(1-196)-Venus

    Plasmid
    #209182
    Purpose
    Mammalian expression of human NKX6-2 cDNA SPAX8-related truncated mutation containing the amino acids 1 to 196; fused with the green fluorescent protein Venus allowing the use of fluorescent based protocols.
    Depositor
    Federico Herrera
    Article
    Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023
    Insert
    NKX6-2 (1-196) fused with Venus (NKX6-2 Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    209182-plasmid-map-sequence-id-413578

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  7. pcDNA3.1 NKX6-2(1-188)-Venus

    Plasmid
    #203714
    Purpose
    SPAX8-related truncated mutation (E189*) containing the amino acids 1 to 188 of human NKX6-2 fused with the Venus fluorescent protein
    Depositor
    Federico Herrera
    Article
    Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023
    Insert
    NKX6-2 (1-188) fused with Venus (NKX6-2 Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    deletion of amino acids 189-277
    Promoter
    CMV
    Available since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    203714-plasmid-map-sequence-id-401198

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  14. Venus-Bik-cb5-pEGFP-C1

    Plasmid
    #177431
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) replaced with Cb5 targeting sequence. Swap made in original Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Bik MBR removed (after position 136) and replaced…
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177431-plasmid-map-sequence-id-348459

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  15. Venus-Bik-delMBR-pEGFP-C1

    Plasmid
    #177432
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) deleted (Bik(1-136)). Compare to full length Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Bik MBR removed (after position 136)
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177432-plasmid-map-sequence-id-348460

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  16. Venus-BclXL-ActA-pEGFP-C1

    Plasmid
    #177410
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-ActA: Bcl-XL with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-XL-ActA, BclX-acta
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177410-plasmid-map-sequence-id-348171

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  17. Venus-BclXL-Cb5-pEGFP-C1

    Plasmid
    #177415
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-Cb5: Bcl-XL with its membrane binding region swapped for Cb5 (ER targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-XL-Cb5, BclX-cb5
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177415-plasmid-map-sequence-id-348201

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  18. Venus-Bcl2-ActA-pEGFP-C1

    Plasmid
    #177419
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-2-ActA, Bcl2-acta, Bcl-acta
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177419-plasmid-map-sequence-id-348305

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  19. Venus-Bcl2-cb5-pEGFP-C1

    Plasmid
    #177422
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-2-Cb5, Bcl2-cb5, Bcl-cb5
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177422-plasmid-map-sequence-id-353685

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  20. Venus-Bik-L61G-pEGFP-C1

    Plasmid
    #177426
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with L61G mutation in BH3 domain to disrupt binding anti-apoptotic proteins. Mutation made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Leucine 61 replaced with glycine
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177426-plasmid-map-sequence-id-348449

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Showing: 581 - 600 of 1659 results