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Plasmid#67275PurposeFor sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell linesDepositorInsertpuromycin resistant gene
UseTagslinked to red flouresent protein gene (katushka)ExpressionMammalianMutationPromoterCAGAvailable sinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Click editor utilizing mSA for template recruitment - pCMV-T7-mSA-nCas9-EcKlenow (JO1391)
Plasmid#217802PurposeVariant CE1 construct with mSA for template recruitment, expressed from CMV or T7 promoters.DepositorInsertmSA-linker-SV40NLS-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Click editor utilizing Cdc13 for template recruitment - pCMV-T7-Cdc13-nCas9-EcKlenow (JO1293)
Plasmid#217796PurposeVariant CE1 construct with Cdc13 telomere binding protein for template recruitment, expressed from CMV or T7 promoters.DepositorInsertCdc13-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Click editor utilizing Cdc13(Y556A) for template recruitment - pCMV-T7-Cdc13(Y556A)-nCas9-EcKlenow (JO1329)
Plasmid#217797PurposeVariant CE1 construct with Cdc13(Y556A) telomere binding protein for template recruitment, expressed from CMV or T7 promoters.DepositorInsertCdc13(Y556A)-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationCdc13(Y556A); nSpCas9(H840A); EcKlenow(-exo;D355A…PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)
Plasmid#217803PurposeVariant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.DepositorInsertmSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSSa13
Plasmid#177371PurposeExpresses red-shifted luciferase under ScPGK1 promoter in Candida glabrataDepositorInsertCgLUCopt-mut2
UseTagsExpressionMutationPromoterScPGK1pAvailable sinceFeb. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSSa16
Plasmid#177372PurposeExpresses red-shifted luciferase under ENO1 promoter in Candida glabrataDepositorInsertCgLUCopt-mut2
UseTagsExpressionMutationPromoterENO1Available sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSSa23
Plasmid#177373PurposeExpresses red-shifted luciferase under TEF1 promoter in Candida glabrataDepositorInsertCgLUCopt-mut2
UseTagsExpressionMutationPromoterTEF1Available sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin- soCoChR-GFP (AAV9)
Viral Prep#107708-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSynapsin- soCoChR-GFP (#107708). In addition to the viral particles, you will also receive purified pAAV-hSynapsin- soCoChR-GFP plasmid DNA. Synapsin-driven somatic CoChR (soCoChR) fused to GFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsGFPAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST (AAV9)
Viral Prep#174007-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST (#174007). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8s and soma-targeted ChrimsonR. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin-FLEX-soCoChR-GFP (AAV9)
Viral Prep#107712-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSynapsin-FLEX-soCoChR-GFP (#107712). In addition to the viral particles, you will also receive purified pAAV-hSynapsin-FLEX-soCoChR-GFP plasmid DNA. Synapsin-driven, Cre-dependent somatic CoChR (soCoChR) fused to GFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsGFP (Cre-dependent)Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-Flpo (AAV Retrograde)
Viral Prep#174378-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-syn-Flpo (#174378). In addition to the viral particles, you will also receive purified pAAV-syn-Flpo plasmid DNA. Syn-driven expression of the recombinase FLPo. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsNoneAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.SF-iGluSnFR.S72A (AAV1)
Viral Prep#106176-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.hSynapsin.SF-iGluSnFR.S72A (#106176). In addition to the viral particles, you will also receive purified pAAV.hSynapsin.SF-iGluSnFR.S72A plasmid DNA. hSynapsin driven SF-iGluSnFR.S72A, glutamate sensor. The S72A mutant has lower affinity for glutamate. Slower on-rate and faster off-rate. Enhanced temporal resolution and spatial resolution. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-iSeroSnFR-Nlgn (AAV9)
Viral Prep#128485-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-CAG-iSeroSnFR-Nlgn (#128485). In addition to the viral particles, you will also receive purified pAAV-CAG-iSeroSnFR-Nlgn plasmid DNA. CAG-driven expression of a fluorescent reporter for serotonin (localized to dendrites). These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Archon1-KGC-GFP-ER2 (AAV8)
Viral Prep#115892-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Syn-Archon1-KGC-GFP-ER2 (#115892). In addition to the viral particles, you will also receive purified pAAV-Syn-Archon1-KGC-GFP-ER2 plasmid DNA. Syn-driven Archon1, an opsin-based fluorescent voltage indicator, fused to EGFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsGFPAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] (AAV8)
Viral Prep#115893-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] (#115893). In addition to the viral particles, you will also receive purified pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] plasmid DNA. Syn-driven, Cre dependent Archon1, an opsin-based fluorescent voltage indicator, fused to EGFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsGFP (Cre-dependent)Available sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEx-iSeroSnFR (AAV9)
Viral Prep#128486-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-CAG-FLEx-iSeroSnFR (#128486). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEx-iSeroSnFR plasmid DNA. CAG driven, Cre-dependent expression of iSeroSnFR. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 (AAV5)
Viral Prep#108422-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 (#108422). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 plasmid DNA. CAG-driven, Cre dependent Archon1, an opsin-based fluorescent voltage indicator, fused to EGFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEGFP (Cre-dependent)Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Antibody#198003-rAbPurposeAnti-HA chimeric recombinant antibody. The hybridoma-derived heavy chain variable sequence and kappa light chain are fused to a rabbit IgG Fc.DepositorRecommended ApplicationsWestern BlotReactivitySource SpeciesRabbitIsotypeIgGTrial SizeAvailable to purchaseAvailable sinceMarch 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits
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Antibody#199805-rAbPurposeAnti-HA chimeric recombinant antibody. The hybridoma-derived heavy chain variable sequence and kappa light chain are fused to a chicken IgY Fc.DepositorRecommended ApplicationsWestern BlotReactivitySource SpeciesChickenIsotypeIgYTrial SizeAvailable to purchaseAvailable sinceApril 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits