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Showing: 61 - 90 of 283 results
  1. pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaC

    Plasmid
    #22931
    Purpose
    Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PDZ binding motif (delta C)
    Depositor
    Insert
    PHLPP1-deltaC (PHLPP1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    PHLPP-deltaC, deletion of PDZ binding domain--the…
    Promoter
    CMV
    Available since
    Feb. 4, 2010
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaC

    Plasmid
    #22931
    Purpose
    Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PDZ binding motif (delta C)
    Depositor
    Insert
    PHLPP1-deltaC (PHLPP1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    PHLPP-deltaC, deletion of PDZ binding domain--the…
    Promoter
    CMV
    Available since
    Feb. 4, 2010
    Availability
    Academic Institutions and Nonprofits only
  3. pHR_PGK_SNIPR_Hinge Notch SNIPR (HNF1A, ALPPL2 scFv)

    Plasmid
    #188385
    Purpose
    Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor
    Depositor
    Insert
    PGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pHR_PGK_SNIPR_Hinge Notch SNIPR (HNF1A, ALPPL2 scFv)

    Plasmid
    #188385
    Purpose
    Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor
    Depositor
    Insert
    PGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pAJE3-E7

    Plasmid
    #214359
    Purpose
    Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins
    Depositor
    Inserts
    2nd generation Tet2 "E7" aminoacyl tRNA synthetase
    Lpp promoted M. jannaschii tRNA
    Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
    Orthogonal "D4II" ColE1 origin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    AmpR and lpp
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pKB224

    Plasmid
    #170014
    Purpose
    Encodes LacI-controlled expression of surface-displayed NC and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    NC
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pALS3-Ma-sfGFP-WT

    Plasmid
    #212119
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Inserts
    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    araC and lpp
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. pKB223

    Plasmid
    #170013
    Purpose
    Encodes LacI-controlled expression of surface-displayed C18G and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    C18G
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. pKB253

    Plasmid
    #170036
    Purpose
    Encodes LacI-controlled expression of surface-displayed K5L7 and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    K5L7
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    May 7, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pKB252

    Plasmid
    #170035
    Purpose
    Encodes LacI-controlled expression of surface-displayed K6L9 and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    K6L9
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. pKB256

    Plasmid
    #170039
    Purpose
    Encodes LacI-controlled expression of surface-displayed Bac2a and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    Bac2a
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. pKB254

    Plasmid
    #170037
    Purpose
    Encodes LacI-controlled expression of surface-displayed H6L9 and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    H6L9
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    Availability
    Academic Institutions and Nonprofits only
  13. pKB224

    Plasmid
    #170014
    Purpose
    Encodes LacI-controlled expression of surface-displayed NC and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    NC
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  14. pKB223

    Plasmid
    #170013
    Purpose
    Encodes LacI-controlled expression of surface-displayed C18G and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    C18G
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
  15. pKB253

    Plasmid
    #170036
    Purpose
    Encodes LacI-controlled expression of surface-displayed K5L7 and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    K5L7
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    May 7, 2022
    Availability
    Academic Institutions and Nonprofits only
  16. pKB252

    Plasmid
    #170035
    Purpose
    Encodes LacI-controlled expression of surface-displayed K6L9 and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    K6L9
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  17. pKB256

    Plasmid
    #170039
    Purpose
    Encodes LacI-controlled expression of surface-displayed Bac2a and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    Bac2a
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  18. pKB254

    Plasmid
    #170037
    Purpose
    Encodes LacI-controlled expression of surface-displayed H6L9 and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    H6L9
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    Availability
    Academic Institutions and Nonprofits only
  19. pKB231

    Plasmid
    #170018
    Purpose
    Encodes LacI-controlled expression of surface-displayed cecropin P1 and PvirK-mNeonGreen reporter
    Depositor
    Inserts
    mNeonGreen
    Cecropin P1
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  20. pBRα

    Plasmid
    #53731
    Purpose
    Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Insert
    αNTD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 61 - 90 of 283 results