pCAT112TAG-SepT
(Plasmid
#34624)
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 34624 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepACYC184
- Backbone size w/o insert (bp) 3200
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Modifications to backboneThe gene encoding chloramphenicol acetyltransferae (CAT) was modified to introduce an amber stop codon at position Asp112 (pCAT112TAG).
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Tetracycline, 25 & 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsPlate separately on Tet and Chlor plates.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametRNA^Sep
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Insert Size (bp)75
- Promoter lpp
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer M13_pUC-fwd (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAT112TAG-SepT was a gift from Jesse Rinehart & Dieter Söll (Addgene plasmid # 34624 ; http://n2t.net/addgene:34624 ; RRID:Addgene_34624) -
For your References section:
Expanding the genetic code of Escherichia coli with phosphoserine. Park HS, Hohn MJ, Umehara T, Guo LT, Osborne EM, Benner J, Noren CJ, Rinehart J, Soll D. Science. 2011 Aug 26;333(6046):1151-4. 10.1126/science.1207203 PubMed 21868676