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Plasmid#52299PurposeReporter for mutated human GPR56 exon 1m promoter activity. It contains a 15-bp deletion in a conserved noncoding element. The mutated human GPR56 e1m promoter was inserted into pGL3E.DepositorInsertADGRG1 adhesion G protein-coupled receptor G1 (ADGRG1 Human)
UseLuciferaseTagsExpressionMutation15-bp deletion in the promoterPromoterHuman GPR56 e1m promoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-PHKG2
Plasmid#23745DepositorInsertPHKG2 (PHKG2 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-BMP2KL
Plasmid#23399DepositorInsertBMP2KL (BMP2KL Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-NME5
Plasmid#23772DepositorInsertNME5 (NME5 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-TWF2
Plasmid#23700DepositorInsertTWF2 (TWF2 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBMN DHFR-HiBiT / Lyt2
Plasmid#179731PurposeExpresses human dihydrofolate reductase (DHFR) with C-terminal HiBiT tag in mammalian cells. This retroviral vector has an IRES-Lyt-2 downstream of the cloning sitesDepositorInserthuman DHFR-HiBiT and IRES-Lyt-2 (DHFR Mouse, Human)
UseRetroviralTagsHiBiTExpressionMutationPromoterLTR/MMLVAvailable sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Myc-BirA*-CAMK2D
Plasmid#221686PurposeMammalian expression of Myc-tagged BirA*-CAMK2D fusion protein for BioIDDepositorInsertCAMK2D (CAMK2D Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
plenti-FLAG-CAMK2D-ED
Plasmid#221700PurposeLentiviral expression of FLAG-tagged human CAMK2D (K43R/D136N) in mammalian cellsDepositorInsertCAMK2D (CAMK2D Human)
UseLentiviralTagsExpressionMutationK43R, D136NPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
plenti-FLAG-CAMK2D
Plasmid#221699PurposeLentiviral expression of FLAG-tagged human CAMK2D in mammalian cellsDepositorInsertCAMK2D (CAMK2D Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SMSr-TEV-Twin-Strep
Plasmid#202530PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase-relate protein (SMSr) in mammalian cellDepositorInsertSAMD8 (SAMD8 Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted stop codon (TGA)PromoterCAG and chicken β-actin promoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
MDM4_Deletion_Upstream_gRNA_2
Plasmid#195135PurposegRNA in a third generation Cas9 vector with GFP, targeting region immediately upstream of MDM4, to be used with MDM4_Deletion_Downstream_gRNA_1/2 for MDM4 deletionDepositorInsertMDM4 Deletion Upstream gRNA 2 (MDM4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
MDM4_Deletion_Upstream_gRNA_1
Plasmid#195134PurposegRNA in a third generation Cas9 vector with GFP, targeting region immediately upstream of MDM4, to be used with MDM4_Deletion_Downstream_gRNA_1/2 for MDM4 deletionDepositorInsertMDM4 Deletion Upstream gRNA 1 (MDM4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLED.CaRPv1
Plasmid#193018PurposeExcitatory/Inhibitory neuron calcium indicator (hSyn promoter, Synrg exon, jRGECO1a/jGCaMP7b)DepositorInsertBichromatic calcium indicator (jGCaMP7b and jRGECO1a)
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLED.RAB
Plasmid#193015PurposePhotoreceptor-specific reporter (CBh promoter, Atp1b2 exon, bichromatic reporter)DepositorInsertBichromatic reporter (EGFP and dsRed-Express2)
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-flag-RAP1B-N17-puro
Plasmid#156169PurposeLentiviral vector for expression of flag-RAP1B-N17, with puromycin selectionDepositorInsertRAP1B (RAP1B Bovine)
UseLentiviralTags2xFLAGExpressionMammalianMutationchanged Serine 17 to AsparaginePromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-RAP2A-WT-puro
Plasmid#156170PurposeLentiviral vector for expression of RAP2A-WT, with puromycin selectionDepositorInsertRAP2A (RAP2A Human)
UseLentiviralTagsExpressionMammalianMutationwild type formPromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-RAP2A-V12-puro
Plasmid#156171PurposeLentiviral vector for expression of RAP2A-V12, with puromycin selectionDepositorInsertRAP2A (RAP2A Human)
UseLentiviralTagsExpressionMammalianMutationchanged Glycine 12 to ValinePromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-RAP2A-N17-puro
Plasmid#156172PurposeLentiviral vector for expression of RAP2A-N17, with puromycin selectionDepositorInsertRAP2A (RAP2A Human)
UseLentiviralTagsnoneExpressionMammalianMutationchanged Serine 17 to AsparaginePromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
t1-405(del30)
Plasmid#166128PurposeExpression of human talin-1 head (residues 1-405) in E. coli. Contains 30 amino acid deletion in F1-loop. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.DepositorInsertT1head1-405(del30) (TLN1 Human)
UseTagsHis6-tag, Xpress-epitope (DLYDDDDK) and enterokin…ExpressionBacterialMutationresidues 1-405, with 30 amino acid deletion in th…PromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
t1-405(T144E,T150E)
Plasmid#166130PurposeFor expression of human talin-1 head (residues 1-405) in E. coli. Includes mutations T144E and T150E. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.DepositorInsertHuT1head1-405(T144E,T150E) (TLN1 Human)
UseTagsHis6-tag, Xpress-epitope (DLYDDDDK) and enterokin…ExpressionBacterialMutationresidues 1-405, mutations T144E and T150EPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only