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Pooled Library#92383PurposeCRISPRaDepositorExpressionMammalianUseCRISPR and LentiviralAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only
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Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set B
Pooled Library#92380PurposeCRISPRaDepositorUseLentiviralAvailable sinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
Compact mismatched sgRNA library for gene titration (human essential genes, CRISPRi)
Pooled Library#136479PurposeExpresses gRNAs for gene titration of ~2400 human genes using CRISPRiDepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Mouse CRISPRa sgRNA library Caprano in backbone XPR_502 (P65 HSF), Set B
Pooled Library#92384PurposeCRISPRaDepositorExpressionMammalianUseCRISPR and LentiviralAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-hUbC-GBX2-CDX124-Cas9-T2A-GFP
Plasmid#192287PurposeExpresses Cas9-T2A-GFP and four sgRNAs: GBX2, CDX1, CDX2, CDX4DepositorInsertUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-GBX2-CDX124-Ef1a-mCherry-Neo
Plasmid#192823PurposeExpresses mCherry and four sgRNAs: GBX2, CDX1, CDX2, CDX4DepositorUseCRISPR and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGE Dicot Genome Editing Kit
Plasmid Kit#1000000084PurposeGolden Gate cloning-based assembly of constructs for CRISPR genome editing in dicotyledonous plants. Consists of âgenome editing recipientâ and âsgRNA shuttleâ vectors.DepositorAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCut plasmid toolkit
Plasmid Kit#1000000118PurposeCas9-based kit in S. cerevisiae for optimizing heterologous gene expression. Cas9-sgRNA (pCut) plasmids for integrating into 23 loci, across all 16 yeast chromosomes.DepositorAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human CRISPR lncRNA activation library (SAM) in lenti sgRNA(MS2)_zeo backbone
Pooled Library#1000000106PurposeHuman CRISPR Synergistic Activation Mediator (SAM) pooled library for the transcriptional activation of lncRNAsDepositorAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in lentiCRISPR v1
Pooled Library#1000000100PurposeCRISPR gRNA knockout pooled library (version 1) for targeting the human genomeDepositorAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
Large-scale mismatched sgRNA library for gene titration (human essential genes, CRISPRi)
Pooled Library#136478PurposeExpresses gRNAs for gene titration of ~2400 human genes using CRISPRiDepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Sabatini/Lander Lab Ras synthetic lethal candidate focused human sgRNA library in lentiCRISPRv1
Pooled Library#92352Purposecontains 6,661 sgRNAs (comprising 499 intergenic control sgRNAs and 6,162 sgRNAs targeting 132 control and candidate Ras synthetic lethal genes at up to 50 sgRNAs per gene)DepositorSpeciesHomo sapiensAvailable sinceJune 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
2X_pX458_pSpCas9(BB)-2A-GFP
Plasmid#172221PurposeCas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.DepositorTypeEmpty backboneUseCRISPRTags3XFLAG and GFPExpressionMammalianMutationPromoterCbhAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAbaMCi-GFP-BsaI_pJMP2748
Plasmid#208888PurposeAcinetobacter baumannii CRISPRi test plasmid (GFP, BsaI cloning site in gRNA cassette).DepositorInsertdCas9, sgRNA expression cassette, sfGFP
UsePir dependent origin of replicationTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCT-tRNA
Plasmid#133813PurposeCRISPR-Cas9 system for genetic manipulation of Candida tropicalisDepositorInsertcassette for the expression of the sgRNA from the Ashbya gossypii RNA pol II TEF1 promoter
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTG131
Plasmid#198640PurposeThermosensitive pSC101-based plasmid expressing a sgRNA under the control of the J23119 promoter and Cas9 under the control of the DAPG-inducible PhlF promoterDepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterpPhlFAvailable sinceJune 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-enCas9-PolI3M-TBD
Plasmid#113077PurposeExpresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI3M-TBD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAWp28
Plasmid#73850PurposepBT264-U6p-{2xBbsI}-sgRNA scaffold-{MfeI}DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSGAb-km
Plasmid#121999PurposeA sgRNA expression plasmid for genome editing in Acinetobacter baumanniiDepositorInsertnone
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-enCas9-PolI5M
Plasmid#113078PurposeExpresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only