Addgene

We narrowed to 1,141 results for: piggyBac

Showing: 561 - 580 of 1141 results

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pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA

Plasmid

#162802

Purpose

STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI-HA

Use

Piggybac

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB_EBFP2

Plasmid

#167873

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Nan Cher Yeo, Alejandro Chavez

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB

Use

CRISPR

Tags

Expression

Mutation

D10A, D839A, H840A, N863A

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-BsmBI

Plasmid

#162801

Purpose

STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pTBL2367 MTK PB empty backbone with no marker

Plasmid

#226675

Purpose

Empty MTK PB cargo backbone with no marker.

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-HOXB5-DD-hygro

Plasmid

#222582

Purpose

PiggyBac transposon plasmid for Shield1-inducible expression of BCL2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HOXB5 (HOXB5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERH-HOXB5

Plasmid

#222584

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HOXB5, with hygromycin resistance

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HOXB5 (HOXB5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-UBB

Plasmid

#222569

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of UBB

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

UBB (UBB Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HDAC6

Plasmid

#222566

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HDAC6

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HDAC6 (HDAC6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-DMRT1

Plasmid

#222571

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DMRT1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

DMRT1 (DMRT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HOXA10

Plasmid

#222539

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HOXA10

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HOXA10 (HOXA10 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HMGB3

Plasmid

#222540

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HMGB3

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HMGB3 (HMGB3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-MEIOC

Plasmid

#222562

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of MEIOC

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

MEIOC (C17orf104 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERH-BCL2

Plasmid

#222583

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of BCL2, with hygromycin resistance

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

BCL2 (BCL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-ESX1

Plasmid

#222563

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of ESX1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

ESX1 (ESX1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-3xFLAG-SMAD1-active

Plasmid

#222579

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of 3xFLAG-SMAD1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

SMAD1 (SMAD1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

S487E, S489E

Promoter

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-3xFLAG-SMAD9-active

Plasmid

#222580

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of 3xFLAG-SMAD9

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

SMAD9 (SMAD9 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

S489E, S491E

Promoter

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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PB-Blast-Dest-LifeAct-mRuby

Plasmid

#194907

Purpose

To generate LifeAct-mRuby expressing stable cell lines with piggybac transposase co-expression and blastocidin selections.

Depositor

Clare Buckley

Article

Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

Insert

LifeAct

Use

Mouse Targeting

Tags

mRuby

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_VPR_mcherry

Plasmid

#167886

Purpose

PiggyBac compatible plasmid expressing dCas9-VPR

Depositor

Nan Cher Yeo, Alejandro Chavez

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-VPR

Use

CRISPR

Tags

Expression

Mutation

D10A, D839A, H840A, N863A

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only
NAX_CAGGS_CAS9_m4_KRAB_mcherry

Plasmid

#167878

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Nan Cher Yeo, Alejandro Chavez

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB

Use

CRISPR

Tags

Expression

Mutation

D10A, D839A, H840A, N863A

Promoter

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only
NAX_CAGGS_CAS9_m4_mcherry

Plasmid

#167870

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Nan Cher Yeo, Alejandro Chavez

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Tags

Expression

Mutation

D10A, D839A, H840A, N863A

Promoter

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only