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pJK295
Plasmid#71561PurposeProduction of Acetobacter aceti 1023 thioredoxin (AaTrx)DepositorInsertthioredoxin
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFLIPmal-40uDelta1YFP
Plasmid#18853DepositorInsertoptimized maltose FRET sensor
UseTagsCFP and YFPExpressionBacterialMutationN227A;PromoterAvailable sinceOct. 23, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCVD433
Plasmid#63756PurposeDerivative of pACYC184 M1uI linkers inserted into EcoRV site inactivating Tc resistance gene. Now TcsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
RCAS IkB alpha M
Plasmid#12407DepositorInsertIkB alpha M (Nfkbia Mouse)
UseRetroviralTagsExpressionMammalianMutationDominant-negative mutant. Many signal transductio…PromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCVD443
Plasmid#66150Purposecloning of EPEC E.coli eae geneDepositorInserteae
UseCloning vectorTagsExpressionMutationPromoternoneAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJK383
Plasmid#72474PurposeProduces Thermoplasma acidophilum citrate synthase with Asp317>Gly mutant (TpCS-D317G)DepositorInsertcitrate synthase
UseTagsExpressionBacterialMutationchanges tryptophan-17 to phenylalanine, tryptopha…PromoterrecAAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
AA498
Plasmid#231119PurposeFragmid fragment: (guide cassette) ABE activity-based selection CD274 positive controlDepositorInsertsgCD274 + ABE splice-targeting sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
AA505
Plasmid#231120PurposeFragmid fragment: (guide cassette) CBE activity-based selection CD274 positive controlDepositorInsertsgCD274 + CBE splice-targeting sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBEST-PrOR1OR2-MGapt-UTR1-deGFP
Plasmid#227648PurposePrOR1OR2-driven expression of MGapt and deGFP for quantifying cell-free RNA and protein synthesis.DepositorInsertMG aptamer (MGapt) and deGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEST-PTet-MGapt-UTR1-deGFP
Plasmid#227647PurposepTet-driven expression of MGapt and deGFP for quantifying cell-free RNA and protein synthesis.DepositorInsertMG aptamer (MGapt) and deGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDK365
Plasmid#134195PurposeRetroviral plasmid with SKAP insertDepositorInsertSKAP Alternate Start- LAP (with G5 hardeneded) (KNSTRN Human)
UseRetroviralTagsLAP (GFP)ExpressionMammalianMutationAlternate Start, G5 siRNA hardenedPromoterAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK605
Plasmid#72530PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); glutaminase subunit (AaPurQ); large subuint (AaPurL)DepositorInsertsformylglycinamidine ribonucleotide synthetase, small subunit
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseTagsExpressionBacterialMutationsynthetic genePromoterT7 and n/aAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK623
Plasmid#72540PurposeProduces Acetobacter aceti 1023 purSyQL associated hypothetical protein; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit (AaOrfY, AaPurQ, AaPurL)DepositorInsertshypothetical protein in purSQL operon
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseTagsExpressionBacterialMutationsynthetic genePromoterT7 and n/aAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK289
Plasmid#71553PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with Lys39>Ala mutant (AaAlr-K39A)DepositorInsertalanine racemase
UseTagsExpressionBacterialMutationchanges lysine-39 to alanine (please see deposito…PromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK501
Plasmid#72246PurposeProduces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp151>Phe mutant (EcPurE1-W151F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)DepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationGln206 to Arg and changes tryptophan-151 to pheny…PromoterT7 and endogenousAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK502
Plasmid#71582PurposeProduces Acetobacter aceti 1023 putative SixA with C-terminal His6 tag (SixAH6)DepositorInsertphosphohistidine phosphatase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK304
Plasmid#72225PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with Ala368>Asp mutant (H6AaAlr-A368D)DepositorInsertalanine racemase
UseTagsExpressionBacterialMutationchanges alanine-368 to aspartatePromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK303
Plasmid#72224PurposeProduction of Acetobacter aceti 1023 alanine racemase (alr) with Ala367>Asp mutant (H6AaAlr-A367D)DepositorInsertalanine racemase
UseTagsExpressionBacterialMutationchanges alanine-367 to aspartatePromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK579
Plasmid#72426PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6), additional unique internal restriction enzyme sitesDepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsHis6ExpressionBacterialMutation4 silent mutations introduce unique internal SpeI…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only
