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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 12331 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMX
- Backbone size w/o insert (bp) 4500
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIkB alpha
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Alt nameIkBa
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Alt nameI kappa B alpha
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SpeciesM. musculus (mouse)
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Entrez GeneNfkbia (a.k.a. Nfkbi)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMX IkB was a gift from Inder Verma (Addgene plasmid # 12331 ; http://n2t.net/addgene:12331 ; RRID:Addgene_12331) -
For your References section:
Suppression of TNF-alpha-induced apoptosis by NF-kappaB. Van Antwerp DJ, Martin SJ, Kafri T, Green DR, Verma IM. Science. 1996 Nov 1. 274(5288):787-9. 10.1126/science.274.5288.787 PubMed 8864120
Map uploaded by the depositor.