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Addgene

pCMX IkB
(Plasmid #12331)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12331 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCMX
  • Backbone size w/o insert (bp) 4500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IkB alpha
  • Alt name
    IkBa
  • Alt name
    I kappa B alpha
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Nfkbia (a.k.a. Nfkbi)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMX IkB was a gift from Inder Verma (Addgene plasmid # 12331 ; http://n2t.net/addgene:12331 ; RRID:Addgene_12331)
  • For your References section:

    Suppression of TNF-alpha-induced apoptosis by NF-kappaB. Van Antwerp DJ, Martin SJ, Kafri T, Green DR, Verma IM. Science. 1996 Nov 1. 274(5288):787-9. 10.1126/science.274.5288.787 PubMed 8864120