These results may also be relevant.
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pET28a(+)::MBP-NL3F10H
Plasmid#141291PurposeMBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertMBP-NLUC3F10H
UseLuciferaseTagsFlag (x3), His (x10), and MBPExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRW006-SM-destination-pro35S-Cas9
Plasmid#104439PurposeDestination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene freeDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoter35SAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
p35S::mCh-HA-P2A-lifeact-mCh
Plasmid#135234Purposeconstitutive expression of 2 mCherry molecules: one tagged with HA and another that binds to actin (control plasmid)DepositorInsertmCh-HA-P2A-lifeact-mCh
UseTagsHA, lifeact, and p2aExpressionPlantMutationPromoter35SAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLBM0090
Plasmid#172824PurposeLevel 2 module containing mCHERRY-nVENUS HSP21-cVENUSDepositorInsert[35S-P19][35S-CTPSSU-mHSP21-nVENUS][35S-CTPSSU-mHSP21-X3FLAGcVENUS][35S-SP_SV40-CFP]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pLBT0160
Plasmid#172812PurposeLevel 1 module containing P19_P1 FDepositorInsertp35S-5UTMV-CDS1_P19-3U+TERNOS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
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T7T3-PacI-Neto1
Plasmid#87672PurposeIn situ probe for mouse Neto1DepositorInsertNeto1 (Neto1 Mouse)
UseTagsExpressionBacterialMutationpartial sequence, (1244 to 2278bp of NM_144946.4)PromoterT7/T3Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pBa.KIF13B
Plasmid#224413PurposeMammalian expression of Kif 13bDepositorInsertKif 13b (Kif13b Mouse)
UseTagsExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-OPHN1
Plasmid#221310PurposeExpression of OPHN1 (pLxIS) in mammalian cells by retroviral transductionDepositorInsertOPHN1 (pLxIS) (Ophn1 Mouse)
UseRetroviralTagsFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
etnppl-pGEMtEasy
Plasmid#192263PurposeMouse Etnppl sequence is inserted in pGEM-T Easy vector.DepositorInsertethanolamine phosphate phospholyase (Etnppl Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(ETNPPL)
Plasmid#192264PurposeCoding sequence of mouse ETNPPL is inserted in pET-15b vectorDepositorInsertethanolamine phosphate phospholyase (Etnppl Mouse)
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLBM0050
Plasmid#172820PurposeLevel 2 module containing mCHERRY-nYFP HSP21-cYFPDepositorInsert[35S-P19][35S-CTPSSU-mCHERRY-3XFLAGnYFP][35S-CTPSSU-mHSP21-cYFPX3HA][35S-SP_SV40-CFP]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBM0040
Plasmid#172819PurposeLevel 2 module containing HSP21-nYFP deltaPTAC5-cYFPDepositorInsert[35S-P19][35S-CTPSSU-mHSP21-3XFLAGnYFP][35S-CTPSSU-deltamPTAC5-cYFPX3HA][35S-SP_SV40-CFP]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBM0030
Plasmid#172818PurposeLevel 2 module containing HSP21-nYFP PTAC5-cYFPDepositorInsert[35S-P19][35S-CTPSSU-mHSP21-3XFLAGnYFP][35S-CTPSSU-mPTAC5-cYFPX3HA][35S-SP_SV40-CFP]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBM0020
Plasmid#172817PurposeLevel 2 module containing HSP21-nYFP HSP21-cYFPDepositorInsert[35S-P19][35S-CTPSSU-mHSP21-3XFLAGnYFP][35S-CTPSSU-mHSP21-cYFPX3HA][35S-SP_SV40-CFP]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBM0060
Plasmid#172821PurposeLevel 2 module containing HSP21-nYFP HSP21-cYFP OEP7-mTRQDepositorInsert[35S-P19][35S-CTPSSU-mHSP21-3XFLAGnYFP][35S-CTPSSU-mHSP21-cYFPX3HA][OEP7_mTRQ]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBM0070
Plasmid#172822PurposeLevel 2 module containing mCHERRY-nYFP HSP21-cYFP OEP7-mTRQDepositorInsert[35S-P19][35S-CTPSSU-mCHERRY-3XFLAGnYFP][35S-CTPSSU-mHSP21-cYFPX3HA][OEP7_mTRQ]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only
