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Plasmid#173151PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj21 (zgc:162160 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 endAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj2a
Plasmid#173127PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj2a (kcnj2a Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj2b
Plasmid#173128PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj2b (kcnj2b Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj3b
Plasmid#173130PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj3b (kcnj3b Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj4
Plasmid#173131PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj4 (kcnj4 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj9
Plasmid#173135PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj9 (kcnj9 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj10a
Plasmid#173137PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj10a (kcnj10a Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj10b
Plasmid#173138PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj10b (LOC100329607 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-APEX2 (GC rich)
Plasmid#115550PurposeMultisite gateway vector for 3' tagging with GC-rich APEX2. Used to make 115563. Parton lab clone HHSDepositorInsertAPEX2 (GC rich)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
C453-E18: attB4-polyhedrinp>-attB5
Plasmid#162977PurposeGateway attB4/attB5 entry clone for baculovirus expression using the AcMNPV polyhedrin promoterDepositorInsertAcMNPV polyhedrin promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
C453-E21: attB4-FerHp>-attB5
Plasmid#162978PurposeGateway attB4/attB5 entry clone for mammalian expression using the human EF1alpha promoterDepositorInserthuman Ferritin heavy chain promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJC8
Plasmid#170757PurposeCo-express in mammalian cells two target proteins C-terminally fused to V5 and FLAG tags respectively, using an internal ribosome entry site and two multi-cloning sites.DepositorTypeEmpty backboneUseTagssite 1- V5 tag and site 2- FLAG tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH198_pE12_hyCal2-eGFP
Plasmid#160411PurposeGateway entry clone containing postsynaptic HyCal component (HyNaC2) for HySyn system fused to eGFPDepositorInsertpDONR221 HyCal2::eGFP
UseOtherTagseGFPExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH200_pE12_hyCal7-eGFP
Plasmid#160413PurposeGateway entry clone containing postsynaptic HyCal component (HyNaC7) for HySyn system fused to eGFPDepositorInsertpDONR221 HyCal7::eGFP
UseOtherTagseGFPExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH201_pE12_hyCal7-mCherry
Plasmid#160414PurposeGateway entry clone containing postsynaptic HyCal component (HyNaC7) for HySyn system fused to mCherryDepositorInsertpDONR221 HyCal7::mCherry
UseOtherTagsmCherryExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH202_pE12_hyCal9-eGFP
Plasmid#160415PurposeGateway entry clone containing postsynaptic HyCal component (HyNaC9) for HySyn system fused to eGFPDepositorInsertpDONR221 HyCal9::eGFP
UseOtherTagseGFPExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH203_pE12_hyCal9-mCherry
Plasmid#160416PurposeGateway entry clone containing postsynaptic HyCal component (HyNaC9) for HySyn system fused to mCherryDepositorInsertpDONR221 HyCal9::mCherry
UseOtherTagsmCherryExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
C417-E10: attB4-polyhedrinp>3xFLAG-nostop-attB1r
Plasmid#162936PurposeGateway attB4/attB1r entry clone for baculovirus expression of 3xFLAG tagged proteins using the polyhedrin promoterDepositorInsertTriple FLAG epitope tag fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C512-E01: attB5r-meGFP-nostop-attB1r
Plasmid#162940PurposeGateway attB5r/attB1r entry clone for generation of N-terminal eGFP fusion proteinsDepositorInsertmonomeric enhanced Green fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
C417-E12: attB4-polyhedrinp>Strep2x2-nostop-attB1r
Plasmid#162937PurposeGateway attB4/attB1r entry clone for baculovirus expression of dual Strep2 tagged proteins using the polyhedrin promoterDepositorInsertHis6 fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only