C417-E12: attB4-polyhedrinp>Strep2x2-nostop-attB1r
(Plasmid
#162937)
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PurposeGateway attB4/attB1r entry clone for baculovirus expression of dual Strep2 tagged proteins using the polyhedrin promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 162937 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDonr233
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHis6 fusion, AcMNPV polyhedrin promoter
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SpeciesSynthetic
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
C417-E12: attB4-polyhedrinp>Strep2x2-nostop-attB1r was a gift from Dominic Esposito (Addgene plasmid # 162937 ; http://n2t.net/addgene:162937 ; RRID:Addgene_162937) -
For your References section:
Combinatorial assembly of clone libraries using site-specific recombination. Wall VE, Garvey LA, Mehalko JL, Procter LV, Esposito D. Methods Mol Biol. 2014;1116:193-208. doi: 10.1007/978-1-62703-764-8_14. 10.1007/978-1-62703-764-8_14 PubMed 24395366