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pIS1 DICER1 long-mut-let-7
Plasmid#21651DepositorInsertDicer 1 (DICER1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationMutant let-7 microRNA binding sitesPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-H391Y
Plasmid#122692PurposeExpress His-tagged PKM2 (H391Y) protein in bacteria.DepositorInsertPyruvate kinase M2 isoform (PKM Human)
UseTags6His-tagExpressionBacterialMutationH391YPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-hB90
Plasmid#18968DepositorInsertBrevican C-terminus (BCAN Human)
UseTagsExpressionMammalianMutationHuman brevican signal peptide aa 1-23 followed by…PromoterAvailable sinceDec. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
MDH1-miR-222-PGK-GFP-Blast
Plasmid#31522DepositorInsertmiR-222 (Mir222 Mouse)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 delta 610-615
Plasmid#19941DepositorInsertcullin 1 delta 610-615 (CUL1 Human)
UseTagsHAExpressionMammalianMutationCUL1 with a ROC1 binding mutantPromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChRger1-TS-EYFP
Plasmid#127243PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.DepositorInsertChRger1-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianMutationPromoterhSyn1Available sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIS1 DICER1 long-mut-all
Plasmid#21653DepositorInsertDicer 1 (DICER1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationMutated let-7 and miR103/107 microRNA binding sit…PromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pIS1 DICER1 long-mut-miR103/107
Plasmid#21652DepositorInsertDicer 1 (DICER1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationMutant miR103/107 microRNA binding sitesPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pProvector_AAV2_586_PLGLAR
Plasmid#129024PurposeRecombinant AAV2 rep/cap plasmid modified to include an 18 amino acid peptide at cap position 586.DepositorInsertRep2/Cap2 with PLGLAR lock at 586
UseAAVTagsExpressionMutationinserted peptide motif (PLGLARGDDDDGPLGLAR) at po…PromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-delta-TMD-VAMP1-IRES-Hygromycin
Plasmid#134866PurposeExpresses EGFP-tagged mutant of VAMP1DepositorInsertVesicle-associated membrane protein 1 (VAMP1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 97-118PromoterEF1aAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
HOXC4 (human) MSCV
Plasmid#8546DepositorInsertHOXC4 (HOXC4 Human)
UseRetroviralTagsExpressionMammalianMutationContains improved ribosomal binding site and NcoI…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 L756A/I757A
Plasmid#19938DepositorInsertcullin 1 L756A/I757A (CUL1 Human)
UseTagsHA2ExpressionMammalianMutationMutation of two adjacent hydrophobic residues, L…PromoterAvailable sinceApril 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEG03
Plasmid#73841PurposeEncodes PhyB917 with a C-terminal histidine tag, for protein expression in Dicty.DepositorInsertPhyB(1-917) with C-term his tag (PIL2 Mustard Weed)
UseDictyostelium discoideum expressionTags6x his tagExpressionMutationPromoterAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
XE223 human myc-Chibby-pCS2+MT
Plasmid#16825DepositorInsertmyc-Chibby (CBY1 Human)
UseXenopus expressionTags6-mycExpressionMutationPromoterAvailable sinceMarch 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
PD-L1-miSFIT-6-C, 16-C
Plasmid#124689PurposeTuning expression of PD-1DepositorInsertPD-L1 (CD274 Human)
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIS1 IMP-1 long-mut-let-7
Plasmid#21642DepositorInsertIGF-II mRNA-binding protein 1 (IGF2BP1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationMutant let-7 microRNA binding sitesPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ERKsub(TA)DD-Venus-NES
Plasmid#84631PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targetedDepositorInsertERKsub(TA)DD-Venus-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationMutated target Thr residue to Ala.PromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLPC-N-Myc-Flag-BirA-hPOT1
Plasmid#166409Purposeexpress BirA-hPOT1 in mammalian cellsDepositorInserthPOT1 (POT1 Human)
UseTagsBirA, flag, and mycExpressionMammalianMutationPromotercmvAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
MDH1-miR-196a-1-PGK-GFP-Blast
Plasmid#31509DepositorInsertmiR-196a1 (Mir196a-1 Mouse)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only
