XE223 human myc-Chibby-pCS2+MT
(Plasmid #16825)

• Depositing Lab: Randall Moon
• Publication: Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

Backbone

• Vector backbone: pCS2+MT
• Backbone size w/o insert (bp): 4352
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: myc-Chibby
• Alt name: cby
• Alt name: chibby
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• Entrez Gene: CBY1 (a.k.a. C22orf2, CBY, Chibby1, HS508I15A, PGEA1, PIGEA-14, PIGEA14, arb1)
• Tag / Fusion Protein:
  • 6-myc

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (unknown if destroyed)
• 3′ cloning site: Xho1 (unknown if destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The full coding sequence as well as 3' UTR of Human Chibby, ~1kb, was amplified by PCR and subcloned into the EcoR1 and Xho1 sites of pCS2+MT, providing 6-myc tags.

Reference: K. Takemaru et al., Nature 422: 905 (2003)