AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)
Plasmid
#60224
Purpose
Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.
Depositor
Inserts
Use
AAV, CRISPR, Cre/Lox, and Mouse Targeting
Tags
Cre-HA and Rluc-P2A
Expression
Mammalian
Mutation
Promoter
EFS, No promoter, and U6
Available since
Oct. 9, 2014
Availability
Academic Institutions and Nonprofits only