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Addgene
Showing: 541 - 560 of 764 results

  1. pcDNA3-AMPKsub-YPet-Kras

    Plasmid
    #84638
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    AMPKsub-YPet-Kras
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    84638-plasmid-map-sequence-id-176882

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  3. SiC-V2-Cas9G7

    Plasmid
    #133043
    Purpose
    Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.
    Depositor
    Sriram Neelamegham
    Article
    Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1
    Insert
    Tet repressor
    Use
    Lentiviral
    Tags
    Cerulean
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    133043-plasmid-map-sequence-id-264424

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  5. pcDNA3-PKAsub(T/A)-YPet-NES

    Plasmid
    #138205
    Purpose
    Encodes negative-control substrate fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub(T/A)-YPet-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138205-plasmid-map-sequence-id-307407

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  6. pcDNA3-PKCsub(T/A)-YPet-NES

    Plasmid
    #138208
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKCsub(T/A)-YPet-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138208-plasmid-map-sequence-id-307411

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  11. pcDNA3-JNKsub(TA)DD-YPet-NES

    Plasmid
    #84634
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    JNKsub(TA)DD-YPet-NES
    Use
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    84634-plasmid-map-sequence-id-176198

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  12. pcDNA3-AMPKsub(TA)-YPet-NES

    Plasmid
    #84636
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    AMPKsub(TA)-YPet-NES
    Use
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    84636-plasmid-map-sequence-id-176880

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  13. FLAG-RFP-UCR1C

    Plasmid
    #67582
    Purpose
    Expresses C-terminal amino acids of UCR1 region of PDE4D3 in mammalian cells
    Depositor
    Graeme Carnegie
    Article
    Wang et al Cell Signal. 2015 May;27(5):908-22. doi:
    Insert
    C-terminus of upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)
    Use
    Tags
    FLAG and TagRFP
    Expression
    Mammalian
    Mutation
    Amino acid residues 80-136 of PDE4D3
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  15. pmCer-RecQL4

    Plasmid
    #211400
    Purpose
    mammalian expression of human RecQL4 N-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    RecQL4 (RECQL4 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211400-plasmid-map-sequence-id-420508

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  16. pmCer-TopBP1

    Plasmid
    #211412
    Purpose
    mammalian expression of human TopBP1 N-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    TopBP1 (TOPBP1 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211412-plasmid-map-sequence-id-420515

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  17. pmCer-RPA32

    Plasmid
    #211385
    Purpose
    mammalian expression of human RPA32 N-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    RPA32 (RPA2 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211385-plasmid-map-sequence-id-420500

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  18. pmCer-C16orf59

    Plasmid
    #211422
    Purpose
    mammalian expression of human C16orf59 N-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    C16orf59 (C16orf59 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211422-plasmid-map-sequence-id-420524

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  19. pmCer-Mcm5

    Plasmid
    #211391
    Purpose
    mammalian expression of human Mcm5 N-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    Mcm5 (MCM5 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211391-plasmid-map-sequence-id-420408

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  20. pmCer-DHX9

    Plasmid
    #211408
    Purpose
    mammalian expression of human DHX9 N-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    DHX9 (DHX9 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211408-plasmid-map-sequence-id-420513

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Showing: 541 - 560 of 764 results