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pDONR223_BIRC6
Plasmid#197974PurposeEntry clone for wt BIRC6DepositorInsertBIRC6 (BIRC6 Human)
UseOtherTagsExpressionMutationL1332VPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II-PEST
Plasmid#204766PurposeGEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)DepositorInsertEGFP-PEST-m6Alinker-DHFR
UseTagsExpressionMutationmutated all RACs in EGFP to RGC/RCCPromoterCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
FKBP-NTEVp(WT)
Plasmid#137831PurposeCytosolic split-TEV protease chain with FKBP Rapamycin sensing domain, N-terminal WT TEV proteaseDepositorInsertFKBP-NTEVp(WT)
UseTags3x FLAG and NESExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
FRB-CTEVp(WT)
Plasmid#137832PurposeCytosolic split-TEV protease chain with FRB Rapamycin sensing domain, C-terminal WT TEV proteaseDepositorInsertFRB-CTEVp(WT)
UseTags3x FLAG and NESExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag TSC2
Plasmid#14129DepositorInsertTSC2 (TSC2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
Pediculus humanus corporis PINK1 (115 - 575)
Plasmid#110750PurposeBacterial expression of PhPINK1 (biochemistry)DepositorInsertPhum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)
UseTagsN-His6-GST-3CExpressionBacterialMutationPromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAV1-mCherry
Plasmid#27705DepositorInsertcaveolin-1 (CAV1 Canis lupus familiaris)
UseTagsmonomeric CherryExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBT277
Plasmid#122608Purposeexpress evoCDA1-BE4max in mammalian cellsDepositorInsertevoCDA1-BE4max
UseTagsExpressionMammalianMutationF23S A123V I195FPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
xCas9 3.7
Plasmid#108379PurposeMammalian expression vector for xCas9 3.7DepositorInsertxCas9 3.7
UseTagsNLSExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VPromoterAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
dFP-mini
Plasmid#120543PurposeExpresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.DepositorInsertde novo fluorescence-activating protein mini (dFP-mini)
UseTags6xHis - TEV cleavageExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
ESCRT-I
Plasmid#17634DepositorInsertUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMET7-GAG-EGFP
Plasmid#80605PurposeMammalian expression vector that expresses HIV-1 GAG with EGFP fused to itDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterSRalphaAvailable sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-mTagBFP-CAAX
Plasmid#75149PurposeMembrane targeted TagBFP (blue) fluorophore for use with 5’ promoters.DepositorInsertmTagBFP-CAAX
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
1307 pcDNA3 GFP PTEN A4
Plasmid#10760DepositorInsertPTEN (PTEN Human)
UseTagsGFPExpressionMammalianMutationA4: S380A, T382A, T383A, S385APromoterAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP1-10-pDEST-C
Plasmid#118369PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsHA-GFP1-10ExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3x-FLAG-hnRNPF
Plasmid#21926DepositorInserthnRNPF (HNRNPF Human)
UseTags3 FlagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 nickase for Split-PE (pJUL2497)
Plasmid#190104PurposeExpresses nickase SpCas9(H840A) for Split-PE useDepositorInsertnSpCas9
UseTagsXTEN-bpNLS and bpNLSExpressionMammalianMutationH840APromoterCMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT120b
Plasmid#122604PurposeBE PACE selection, express gIII, luxAB and degron-GTCCA guide RNADepositorInsertgIII, luxAB, guide RNA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT44c
Plasmid#122600PurposeBE PACE selection, express C-intein-Cas9-ugi fusionDepositorInsertintein-dCas9-ugi fusion
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only
