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Plasmid#176846Purposelentiviral vector for doxycycline-inducible human YAP1 knockdownDepositorInsertYAP1-Sh1 (YAP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1181 - pmU6(loxP-STOP-loxP) BbsI gRNA
Plasmid#113160PurposeA plasmid for cloning Cre-dependent guide RNAs using a modified mouse U6 promoter containing loxPDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromotermU6Available sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-MCS-MED1long-IDR-YFP-NLS
Plasmid#145276PurposeMammalian Expression of Nuclear MED1 long IDR-YFP with SV40 NLSDepositorInsertMED1-IDR long (MED1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
t1-405
Plasmid#166127PurposeFor expression of human talin-1 head (residues 1-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.DepositorInsertTln1Head (1-405) (TLN1 Human)
UseTagsHis6-tag, Xpress-epitope (DLYDDDDK) and enterokin…ExpressionBacterialMutationresidues 1-405 onlyPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYX233-b2-DHFR
Plasmid#163759PurposeGal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)-DHFRDepositorInsertb2-DHFR (Dhfr Mouse, Budding Yeast)
UseTagsExpressionYeastMutationAmino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…PromoterGAL1Available sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-TRE3G-hYAP1-CMV-mCherry-T2A-Puro
Plasmid#176850Purposelentiviral vector for doxycycline-inducible YAP1 overexpressionDepositorInserthYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationfull length YAP1PromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-YAP1-shRNA2
Plasmid#176847Purposelentiviral vector for doxycycline-inducible human YAP1 knockdownDepositorInsertYAP1-Sh2 (YAP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
retro-gfp-puro vector
Plasmid#58249PurposeRetroviral expression vector encoding bicistronic EGFP and Puromycin cassetteDepositorInsertEGFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Twin-Strep-TEV-DGKζ
Plasmid#202531PurposeExpress N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human diacylglycerol kinase ζ (DGKζ) in mammalian cellDepositorInsertDGKZ (DGKZ Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationPromoterCAG and chicken β-actin promoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MEKDD
Plasmid#31202DepositorInsertMAPKK1 (MAP2K1 Human)
UseGateway donor vectorTagsExpressionMutationS218D S222DPromoterAvailable sinceJune 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
61426-hU6-shmFmr1-EF1a-mCherry
Plasmid#157859PurposeExpresses a shRNA targeting mouse Fmr1 geneDepositorInsertFmr1 (Fmr1 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSSRB069_pAC8-hsDDB1dB
Plasmid#218790PurposeInsect cell expression vector for 6xHis-TEV-hsDDB1∆BDepositorInsertDNA damage-binding protein 1 (DDB1 Human)
UseTags6xHis-TEVExpressionInsectMutationDeleted amino acids 396-705 and replaced with GNG…PromoterpolyhedrinAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGem-sHyper:sAPX
Plasmid#84738PurposeSimultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cellsDepositorInsertsUseTagsnuclear localisation signals from the coding sequ…ExpressionPlantMutationPromoterpFMV/p35SAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-H-RAS V12
Plasmid#31201DepositorInsertH-Ras (HRAS Human)
UseGateway donor vectorTagsExpressionMutationG12V constitutively activePromoterAvailable sinceJuly 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGem-nHyPer:sAPX
Plasmid#84735PurposeSimultaneous expresion of stromal HyPer2 and stromal Ascorbate peroxidase (APX) in plant cellsDepositorInsertsUseTagsnuclear localisation signals from the coding sequ…ExpressionPlantMutationPromoterFMV/35SAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-PLXNB2-shRNA1
Plasmid#98399PurposeLentivirus for expression of shRNA1 against human PLEXIN-B2 (Dox-inducible)DepositorInsertPlexin-B2 (PLXNB2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
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cryS96
Plasmid#67519PurposeαB-crystallin minipeptide fused to ELP (S96).DepositorInsertmini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Synthetic, Human)
UseTagselastin-like polypeptide (ELP) S96ExpressionBacterialMutationmini-αB-crystallin contains residues 73–92 of aB-…PromoterT7Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-Ago2-FDM3
Plasmid#72210PurposeMammalian expression of aa 227-860 of human Ago2DepositorInsertargonaute RISC catalytic component 2 (AGO2 Human)
UseTagsFLAGExpressionMammalianMutationΔ 1-226PromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-Ago2-FDM1
Plasmid#72208PurposeMammalian expression of aa 1-372 of human Ago2DepositorInsertargonaute RISC catalytic component 2 (AGO2 Human)
UseTagsFLAGExpressionMammalianMutationΔ 373-860PromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only