cryS96
(Plasmid #67519)

• Purpose: αB-crystallin minipeptide fused to ELP (S96).
• Depositing Lab: Andrew MacKay
• Publication: Wang et al J Control Release. 2014 Oct 10;191:4-14. doi: 10.1016/j.jconrel.2014.04.028. Epub 2014 Apr 26.

Backbone

• Vector backbone: pET25b (+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5547
• Total vector size (bp): 7053
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mini-αB-crystallin fused to ELP S96 (VPGSG)
• Alt name: cry, mini-αB-crystallin
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1506
• Mutation: mini-αB-crystallin contains residues 73–92 of aB-crystallin
• Entrez Gene: CRYAB (a.k.a. CMD1II, CRYA2, CTPP2, CTRCT16, HEL-S-101, HSPB5, MFM2)
• Promoter: T7
• Tag / Fusion Protein:
  • elastin-like polypeptide (ELP) S96 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry