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Plasmid#47943PurposeCan be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequencesDepositorInsertguide RNA
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJW1310
Plasmid#61253PurposeTemplate for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusionDepositorInsertU6 promoter
UseCRISPR; Cloning templateTagsExpressionMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
px330 SUZ12 - pDY083
Plasmid#171109PurposeExpresses Cas9 and an sgRNA targeting SUZ12DepositorInsertCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
YN2_1_IL50_HOlocus
Plasmid#194426PurposeExpresses Cas9 and sgRNA cassettes for targeting the HO locus in yeast Saccharomyces cerevisiaeDepositorInsertCas9
UseTagsExpressionYeastMutationNAPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJF131B
Plasmid#113326PurposepTet-W108 scRNA.b2, pTet-RR2-sgRNADepositorInsertW108 scRNA_MS2 and sgRNA_RR2
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMB60
Plasmid#47941PurposeCan be used to generate synthetic guide in vitro from T7 promotorDepositorInsertsynthetic guide RNA
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAN-PA2-RFP
Plasmid#62248Purposereporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1DepositorInsertmRFP
UseTagsExpressionBacterialMutationPromoterpA2Available sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
U6>XX
Plasmid#87542PurposeComponent 1 of 2 for U6>sgRNA cassette OSO-PCRDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJan. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
sgPal1 (p2Tol-U6-sgPal1-HygR)
Plasmid#71483PurposeExpresses palindromic sgRNA for use in self-cloning CRISPRDepositorInsertsgPal1
UseTagsExpressionMutationPromoterU6Available sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-7SK-Blast
Plasmid#183620PurposelentiCRISPRv2 derivative with modified 7SK promoter driving sgRNA and encoding Blasticidin resistance. Compatible with inserts designed for lentiCRISPRv2.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterModified 7SKAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAN-PA1-RFP
Plasmid#62247Purposereporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1DepositorInsertmRFP
UseTagsExpressionBacterialMutationPromoterpA1Available sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
YN2_1_LT84_RPL13A
Plasmid#177712PurposeExpresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiaeDepositorInsertCas9
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAN-PTet-YFP
Plasmid#62245Purposeused to characterize the promoter activities of PTet for dCas9 and sgRNA response functions.DepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN-PA3-RFP
Plasmid#62249Purposereporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1DepositorInsertmRFP
UseTagsExpressionBacterialMutationPromoterpA3Available sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBC2101-reci
Plasmid#117170PurposeU6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 with NLSDepositorInsertreci_wtCas9 with SV40 NLS
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-YFP
Plasmid#62246Purposeused to characterize the promoter activities of PBAD for dCas9 and sgRNA response functions.DepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
LBJJ383
Plasmid#117517PurposePCR template for sgRNA classic with polyT as terminatorDepositorInsertBpiI:ACTA:pU6-26:sgRNA:polyT:TTAC:BpiI
UseCRISPRTagsExpressionPlantMutationPromoterAtU6-26Available sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa VP64 Afp
Plasmid#99697PurposeExpresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Afp promoter, vector allows for activation of mouse Afp, can be packaged and delivered as AAVDepositorInsertdCas9 and gRNA targeting Afp (Afp Synthetic)
UseAAV and CRISPRTagsVP64ExpressionMutationdead Cas9PromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)
Plasmid#225755PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpy
Plasmid#220184PurposesgRNA expression vector - promoterless backboneDepositorInsertpromoterless backbone
UseCRISPRTagsExpressionBacterialMutationPromoterpromoterlessAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only