Addgene

We narrowed to 14,979 results for: grna

Showing: 5521 - 5540 of 14979 results

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pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

Plasmid

#165450

Purpose

Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

Depositor

Elvir Becirovic

Article

Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

Inserts

dCas9N-IntN
3x Opn1mw promoter-targeting sgRNAs

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 and short human rhodopsin promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA

Plasmid

#158972

Purpose

Vector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP64

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

pMecp2

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pEJS654 All-in-One AAV-sgRNA-hNmeCas9

Plasmid

#112139

Purpose

Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.

Depositor

Erik Sontheimer

Article

Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:

Inserts

sgRNA scaffold
Human-codon-optimized NmeCas9

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U1a and U6

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEM047 [T7-assisted CROPseq sgRNA expression vector]

Plasmid

#224899

Purpose

Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA

Depositor

Thomas Norman

Article

Metzner et al bioRxiv [Preprint]. 2024 Jul 27:2024.07.

Inserts

eGFP
LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-dSaCas9-KRAB-pU6-sgRNA

Plasmid

#158988

Purpose

Vector E encodes pAAV-pMecp2-dSaCas9-KRAB-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-KRAB

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

pMecp2

Available since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-U6-gRNA-UbC-eGFP-P2A-Bsr

Plasmid

#83925

Purpose

Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

eGFP
Bsr

Use

CRISPR and Lentiviral

Tags

P2A

Expression

Mutation

Promoter

hUbC

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA

Plasmid

#55201

Purpose

Plasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues.

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

dsRed2

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

Plasmid

#128749

Purpose

Expresses control gRNA

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR

Plasmid

#162334

Purpose

Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

dSaCas9-KRAB

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-U6-gRNA-UbC-DsRed-P2A-Bsr

Plasmid

#83919

Purpose

Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

DsRed-Express2
Bsr

Use

CRISPR and Lentiviral

Tags

P2A

Expression

Mutation

Promoter

hUbC

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA1

Plasmid

#117405

Purpose

SpyCas9 sgRNA 1 targeting TLR2.0

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

SpyCas9 sgRNA targeting TLR 2.0

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLQ-Pxyl/tet-cas9-Pj23119-sgRNA

Plasmid

#92121

Purpose

CRISPR-Cas9 based efficient genome editing in S. aureus

Depositor

Sheng Yang

Article

Liu et al Acta Biochim Biophys Sin (Shanghai). 201

Insert

Cas9-Pxyltet-sgRNA-pj23119

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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Pzac2.1 U6-Fermt2 sgRNA1, 2, 3_gfaABC1D mcherry SV40

Plasmid

#179117

Purpose

Expresses Fermt2 sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

Depositor

Baljit Khakh

Article

Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

Insert

Fermt2 sgRNA

Use

AAV

Tags

Expression

Mutation

Promoter

U6

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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SLBP non-targeting gRNA lambda phage

Plasmid

#132548

Purpose

expresses gRNA for SLBP-based CIRTS

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

gRNA SLBP-based CIRTS

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6 promoter

Available since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pRRL-gRNA(anti-cGAS)-Cas9-Puro

Plasmid

#130908

Purpose

gRNA targeting human cGAS; Cas9 insert; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

Cas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)sgRNA_CAG-Cas9-venus-bpA

Plasmid

#86986

Purpose

For cloning and expression of sgRNA together with expression of a Cas9-Venus fusion protein

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

Cas9-venus

Use

Tags

Cas9-Venus fusion protein

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only
TBP6.7 non-targeting gRNA lambda phage

Plasmid

#132547

Purpose

expresses gRNA for TBP-based CIRTS

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

gRNA TBP-based CIRTS

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6 promoter

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFP

Plasmid

#162337

Purpose

Lentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

S. pyogenes sgRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6; mU6

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

Plasmid

#113039

Purpose

AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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U6-Slc1a3 sgRNA; EF1a-dCas9-KRAB-GFP

Plasmid

#194283

Purpose

EF1a-dCas9-KRAB-GFP with Slc1a3 sgRNA

Depositor

Elizabeth Kirby

Article

Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

Insert

dCas9-KRAB-GFP

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mutation

Promoter

EF1a

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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